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DOI :
10.3791/62753-v
October 29th, 2021
Chapters
0:04
Introduction
0:38
Cell Preparation
2:05
Lysate Preparation
4:13
Cell-Free Gene Expression
4:47
Results: Green Fluorescent Protein (GFP) Expression Using the Autolysate
5:18
Conclusion
이 프로토콜은 세포 없는 유전자 발현을 위한 세균성 용해를 생성하는 신속하고 간단한 방법을 설명하며, 대장균의 엔지니어링 된 균주를 사용하고 표준 실험실 장비만 필요합니다.
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