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DOI :
10.3791/62791-v
December 25th, 2021
Chapters
0:08
Introduction
1:17
Cloning of Genes in Entry and Destination Vectors
2:01
Growth Conditions for Nicotiana benthamiana Plants
3:05
Prepare Bacterial Strains for Agroinfiltration
6:20
Prepare Slides for Fluorescence Visualization
6:45
Fret-Flim Analysis Using a Confocal Laser Scanning Microscope
10:51
Fret-Flim Analysis Using a Confocal Laser Scanning Microscope: The Tripartite Interaction
12:31
Representative Results
13:42
Conclusion
여기서 우리는 BiFC 기반 FRET-FLIM 분석법에 의한 형광 태그 단백질을 사용하여 3개의 단백질 파트너 간의 삼차 복합 형성을 시각화하는 방법을 제시합니다. 이 방법은 생체 내에서단백질 단백질 상호 작용 복합체를 연구하는 데 유용합니다.
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