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DOI :
10.3791/62797-v
•
7:21 min
July 30th, 2021
Chapters
0:04
Introduction
0:52
Inject Embryos with RNA
2:03
Dechorionate Embryos
3:29
Cut Explants
4:35
Chimeric Explants
5:45
Results: Naïve Blastoderm Explants From Zebrafish Embryos
6:41
Conclusion
斑马鱼胚泡外植体是通过从早期胚胎内的内源性信号中心分离胚胎细胞而产生的,产生相对幼稚的细胞簇,易于操纵和 离体培养。本文提供了制造这种外植体的说明,并通过询问原肠胚形成过程中节点信号的作用来证明它们的效用。
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