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DOI :
10.3791/62837-v
•
8:12 min
September 17th, 2021
Chapters
0:04
Introduction
0:45
Gradient Layering
2:40
Erythrocyte Sedimentation
3:45
Residual RBC Lysis
5:06
Quality Determination of Isolated Neutrophil
5:44
Results: Neutrophil Yield and Quality Estimation
7:34
Conclusion
该协议详细介绍了从全血,黄褐色涂层或白细胞分离膜中性粒细胞的方法,从而实现良好的产量,高纯度和最小的细胞活化。我们利用梯度纯化,红细胞(RBC)沉降和红细胞裂解来获得高质量/纯度的中性粒细胞制剂。
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