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DOI :
10.3791/62837-v
•
8:12 min
September 17th, 2021
Chapters
0:04
Introduction
0:45
Gradient Layering
2:40
Erythrocyte Sedimentation
3:45
Residual RBC Lysis
5:06
Quality Determination of Isolated Neutrophil
5:44
Results: Neutrophil Yield and Quality Estimation
7:34
Conclusion
이 프로토콜은 전혈, 버피 코트 또는 백혈구 막에서 호중구를 분리하여 좋은 수율, 높은 순도 및 최소한의 세포 활성화를 달성하는 방법을 자세히 설명합니다. 그라데이션 정제, 적혈구(RBC) 퇴적물 및 RBC 용액을 활용하여 고품질/순도 호중구 제제를 획득합니다.
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