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DOI :
10.3791/62843-v
•
9:55 min
August 12th, 2021
Chapters
0:04
Introduction
0:30
Conjugation to Generate the Transposon Mutant Library
3:32
Mutant Pool Infection on Beetle Eggs
4:43
Sequencing Library Preparation
8:34
Results: Transposon-insertion Sequencing as a Tool to Elucidate Bacterial Colonization Factors
9:11
Conclusion
这是一种适应方法,用于识别 伯克霍尔德里亚 有益共生的候选昆虫殖民因子。甲虫宿主感染了通过转波突变产生的随机突变库,殖民化后的库复杂性被比作 体外生长的对照。
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