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DOI :
10.3791/62865-v
•
9:56 min
August 31st, 2021
Chapters
0:05
Introduction
0:58
Single-cell Preparation and Staining
2:32
Optical Trapping (OT) Chamber Spacing and Optical Tweezers Start-up
3:58
Alignment of the Optical Force Sensor
5:17
Performing the Nucleus Indentation Experiments
7:22
Results: Measurement of the Forcesand Material Properties of the Cell Nucleus Inside Zebrafish Embryo
9:17
Conclusion
여기서, 우리는 광학 트랩에 의한 직접 힘 측정을 통해 3차원 감금에서 격리된 배아 제브라피쉬 세포의 세포내 기계적 특성을 조사하는 프로토콜을 제시한다.
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