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DOI :
10.3791/62866-v
July 22nd, 2021
Chapters
0:05
Introduction
0:36
Media Growth
1:35
Cell Harvest
2:34
Extract Preparation
3:43
Cell-Free Protein Synthesis and Quantification of the Reporter Protein, Super Folder Green Fluorescent Protein (sfGFP)
4:46
Results: Comparison Between Cell Extracts Obtained from Cell-Free Autoinduction Media Grown to Optical Densities of 2.5 and 10
5:27
Conclusion
この研究は、 大腸菌(E.coli )からの細胞抽出物の調製とそれに続く無細胞タンパク質合成(CFPS)反応を24時間未満で記載する。無細胞自己誘導(CFAI)プロトコルの説明は、研究者の監督を減らし、得られる細胞抽出物の量を増やすために行われた改善を詳述する。
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