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DOI :
10.3791/62901-v
•
6:00 min
September 20th, 2021
Chapters
0:04
Introduction
0:41
Tissue Preparation
1:41
Tissue Homogenization and Cell Lysis
3:11
Nuclei Purification
4:40
Results: Single‐Nucleus RNA‐Sequencing Using Nuclei Isolated from Adult Mouse Kidney
5:30
Conclusion
ここでは、髄質腎細胞タイプの表現を改善し、酵素組織解離による遺伝子発現アーティファクトを回避する凍結マウス腎臓から高品質の核を単離するためのプロトコルを提示します。
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