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DOI :
10.3791/62901-v
•
6:00 min
September 20th, 2021
Chapters
0:04
Introduction
0:41
Tissue Preparation
1:41
Tissue Homogenization and Cell Lysis
3:11
Nuclei Purification
4:40
Results: Single‐Nucleus RNA‐Sequencing Using Nuclei Isolated from Adult Mouse Kidney
5:30
Conclusion
여기에서 우리는 수질 신장 세포 유형의 표현을 개선하고 효소 조직 해리로 인한 유전자 발현 아티팩트를 방지하는 냉동 마우스 신장에서 고품질 핵을 분리하는 프로토콜을 제시합니다.
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