All-optisk mekanobiologi Forhør af ja-associeret protein i humane kræft og normale celler ved hjælp af et multifunktionelt system
December 20th, 2021
•Qin Luo1*, Miao Huang2*, Chenyu Liang2, Justin Zhang3, Gaoming Lin1, Sydney Yu1, Mai Tanaka4,5, Sharon Lepler4,5, Juan Guan5,6,7, Dietmar Siemann4,5, Xin Tang2,5
1Department of Electrical and Computer Engineering, Herbert Wertheim College of Engineering, University of Florida, 2Department of Mechanical and Aerospace Engineering, Herbert Wertheim College of Engineering, University of Florida, 3Department of Electrical and Computer Engineering, University of California, 4Department of Radiation Oncology, College of Medicine, University of Florida, 5UF Health Cancer Center, University of Florida, 6Department of Physics, College of Liberal Arts and Sciences, University of Florida, 7Department of Anatomy and Cell Biology, College of Medicine, University of Florida
Dette papir præsenterer en detaljeret trinvis protokol om, hvordan man bruger et integreret multifunktionelt og brugerprogrammerbart system, der muliggør automatisk flerkanalsbilleddannelse og mekanobiologisk analyse for at belyse mekanofølsomheden af Ja-associeret protein (YAP).
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved