Brain Dissociation and Magnetic Microglial Isolation
4:23
Phagocytic Assay
5:03
Results: Phagocytic Activity and Magnetic Isolation of Microglia In Vitro
6:49
Conclusion
Transcript
This protocol allow us to culture microglia in conditions that closely mimics the in vivo characteristics. Using magnetic cell sorting technology, our protocol allow us to stimulate microglia only two day in vitro, without using any serum in the c
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Primary microglia cultures are commonly used to evaluate new anti-inflammatory molecules. The present protocol describes a reproducible and relevant method to magnetically isolate microglia from neonate pups.