JoVE Logo
Faculty Resource Center

Sign In

One Mouse, Two Cultures: Isolation and Culture of Adult Neural Stem Cells from the Two Neurogenic Zones of Individual Mice

DOI :

10.3791/51225-v

9:52 min

February 25th, 2014

February 25th, 2014

36,498 Views

1Center for Regenerative Therapies Dresden, Technische Universität Dresden, 2German Center for Neurodegenerative Diseases (DZNE) Dresden

Here we describe a detailed protocol for the simultaneous generation of neural precursor cell cultures, as either adherent monolayers or neurospheres, from the subventricular zone and dentate gyrus of individual adult mice.

Tags

Neurosphere Assay

-- Views

Related Videos

article

Isolation and Expansion of the Adult Mouse Neural Stem Cells Using the Neurosphere Assay

article

Isolation and Culture of Neural Crest Stem Cells from Human Hair Follicles

article

Detection of Microregional Hypoxia in Mouse Cerebral Cortex by Two-photon Imaging of Endogenous NADH Fluorescence

article

Isolation and Culture of Neural Crest Cells from Embryonic Murine Neural Tube

article

Growing Neural Stem Cells from Conventional and Nonconventional Regions of the Adult Rodent Brain

article

Cell Sorting of Neural Stem and Progenitor Cells from the Adult Mouse Subventricular Zone and Live-imaging of their Cell Cycle Dynamics

article

External Excitation of Neurons Using Electric and Magnetic Fields in One- and Two-dimensional Cultures

article

Isolation and Culture of Adult Neural Stem Cells from the Mouse Subcallosal Zone

article

Isolation and Culture of Embryonic Mouse Neural Stem Cells

article

The Detection of 5-Hydroxymethylcytosine in Neural Stem Cells and Brains of Mice

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved