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DOI :
10.3791/62985-v
November 16th, 2021
Chapters
0:04
Introduction
1:17
Surgical Excision and Tissue Preparation
2:11
Preparation of Single-Cell Suspension
4:12
Surface Antibody Staining
5:25
Cell Fixation and Intracellular Staining
6:46
Results: Gating Strategy
9:10
Conclusion
この研究では、マウス呼吸器系の免疫集団を単離するための効果的で再現可能なプロトコルを提示する。また、9色ベースのフローサイトメトリーパネルを使用して、健康なマウスの肺に存在するすべての自然免疫細胞および適応免疫細胞を同定する方法も提供しています。
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