Name: pyrosequencing: a simple method for accurate genotyping
Uploaded: 2008-01-08T13:47:00
Duration: 13 min 6 s
Description: Watch this Scientific Journal Video about Pyrosequencing: A Simple Method for Accurate Genotyping at JoVE.com

这个实验方法的完整的文本<a href="http://www.springerprotocols.com/Abstract/doi/10.1385/1-59745-377-3:39">协议，</a>在斯普林格协议。

The authors have nothing to disclose.

pyrosequencing: a simple method for accurate genotyping

完成人类基因组计划所提供的信息丰富的第一手的药理学研究的好处。有了这样一个大量的可用数据，基因分型方法的爆炸。焦磷酸测序（R）是其中一个最彻底而又简单的方法用于分析基因多态性。它还具有识别能力，三等位基因，INDELS，短重复序列多态性，同时与甲基化或样本评估确定的等位基因百分比。此外，还有一个标准化的控制序列，提供了内部质量控制。此方法具有快速，高效的单核苷酸多态性的评价，包括很多临床相关基因多态性。焦磷酸测序的技术和方法进行了解释。

Watch this Scientific Journal Video about Pyrosequencing: A Simple Method for Accurate Genotyping at JoVE.com

Pyrosequencing: A Simple Method for Accurate Genotyping

焦磷酸测序（R）是其中一个最彻底而又简单的方法用于分析基因多态性。此方法具有快速，高效的单核苷酸多态性的评价，包括很多临床相关基因多态性。焦磷酸测序的技术和方法进行了解释。

焦磷酸测序：一个准确的基因分型的简单方法

Pharmacogenetic research benefits first-hand from the abundance of information provided by the completion of the Human Genome Project. With such a ...

Research

JoVE Journal

Biology

A PCR-based Genotyping Method to Distinguish Between Wild-type and Ornamental Varieties of Imperata cylindrica

A PCR-based Genotyping Method to Distinguish Between Wild-type and Ornamental Varieties of Imperata cylindrica

一个基于PCR的基因分型方法来区分野生型和观赏品种白茅</em

Wild-type I. cylindrica (cogongrass) is one of the top ten worst invasive plants in the world, negatively impacting agricultural and natural resources in ...

科研

生物学

Ice-Cap: A Method for Growing Arabidopsis and Tomato Plants in 96-well Plates for High-Throughput Genotyping

Ice-Cap: A Method for Growing Arabidopsis and Tomato Plants in 96-well Plates for High-Throughput Genotyping

冰帽：为发展方法拟南芥和番茄植株在96孔板高通量的基因分型

It is becoming common for plant scientists to develop projects that require the genotyping of large numbers of plants. The first step in any genotyping ...

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FSL，构造一个简单方法修改细胞/病毒粒子表面的生物标志物的范围：在不影响他们的生存能力

The ability to modify/visualize biological surfaces, and then study the modified cell/virion in a range of in vitro and in vivo environments is essential ...

A Simple Method for Imaging Arabidopsis Leaves Using Perfluorodecalin as an Infiltrative Imaging Medium

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一个影像的简单方法拟南芥叶浸润成像介质中使用全氟萘烷

The problem of acquiring high-resolution images deep into biological samples is widely acknowledged1. In air-filled tissue such as the spongy mesophyll of ...

Pyrosequencing for Microbial Identification and Characterization

焦磷酸测序的微生物鉴定和表征

Pyrosequencing is a versatile technique that facilitates microbial genome sequencing that can be used to identify bacterial species, discriminate ...

Infinium Assay for Large-scale SNP Genotyping Applications

Infinium检测的大型SNP基因分型应用

Genotyping variants in the human genome has proven to be an efficient method to identify genetic associations with phenotypes. The distribution of ...

An Improved Method for Accurate and Rapid Measurement of Flight Performance in Drosophila

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在对飞行性能的准确，快速测定方法的改进<em&gt;果蝇</em

Drosophila has proven to be a useful model system for analysis of behavior, including flight. The initial flight tester involved dropping flies into an ...

Simple Method for Fluorescence DNA In Situ Hybridization to Squashed Chromosomes

Simple Method for Fluorescence DNA In Situ Hybridization to Squashed Chromosomes

对于荧光DNA的简易方法<em&gt;原位</em&gt;杂交来压扁染色体

DNA in situ hybridization (DNA ISH) is a commonly used method for mapping sequences to specific chromosome regions. This approach is particularly ...

qPCRTag Analysis - A High Throughput, Real Time PCR Assay for Sc2.0 Genotyping

qPCRTag分析 - 高通量，实时定量PCR检测方法的基因分型Sc2.0

The Synthetic Yeast Genome Project (Sc2.0) aims to build 16 designer yeast chromosomes and combine them into a single yeast cell. To date one synthetic ...

Filtration Isolation of Nucleic Acids:  A Simple and Rapid DNA Extraction Method

核酸的分离过滤：一种简单快速的DNA提取方法

FINA, filtration isolation of nucleic acids, is a novel extraction method which utilizes vertical filtration via a separation membrane and absorbent pad ...