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DOI :
10.3791/63049-v
•
11:11 min
October 8th, 2021
Chapters
0:05
Introduction
1:09
Stereotaxic Injection of Virus in the Medial Prefrontal Cortex (mPFC) of the Mouse Brain
2:28
Gradient Index (GRIN) Lens Implantation in the Medial Prefrontal Cortex (mPFC) of the Mouse Brain
6:47
Affixing Miniscope Holder (Base) to the Mouse Skull
7:53
Miniscope Mounting and In Vivo Ca2+ Imaging
9:37
Results: In Vivo Calcium Imaging Using a Miniscope
10:32
Conclusion
インビボカルシウムイメージングのミニスコープは、自由に行動するマウスのニューロンダイナミクスとマイクロサーキットを研究するための強力な技術です。このプロトコルは、ミニスコープを使用して良好なin vivoカルシウムイメージングを達成するために脳手術を行うことを記載しています。
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