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DOI :
10.3791/63049-v
•
11:11 min
October 8th, 2021
Chapters
0:05
Introduction
1:09
Stereotaxic Injection of Virus in the Medial Prefrontal Cortex (mPFC) of the Mouse Brain
2:28
Gradient Index (GRIN) Lens Implantation in the Medial Prefrontal Cortex (mPFC) of the Mouse Brain
6:47
Affixing Miniscope Holder (Base) to the Mouse Skull
7:53
Miniscope Mounting and In Vivo Ca2+ Imaging
9:37
Results: In Vivo Calcium Imaging Using a Miniscope
10:32
Conclusion
Miniscope in vivo 칼슘 이미징은 자유롭게 행동하는 마우스에서 신경 역학과 미세 회로를 연구하는 강력한 기술입니다. 이 프로토콜은 미니 스코프를 사용하여 좋은 생체 내 칼슘 이미징을 달성하기 위해 뇌 수술을 수행하는 것을 설명합니다.
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