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DOI :
10.3791/63060-v
November 5th, 2021
Chapters
0:04
Introduction
0:50
Validating In Vitro Luciferase Activity
1:55
Injecting Mice with GFP+ Luc+ Cells
2:50
Measuring the Luciferase Levels in GFP+ Luc+ Mice
4:43
Results: Detection of Tumor Development and Metastatic Colonization In Real-Time
6:05
Conclusion
여기에서, 우리는 다양한 유방암 세포주에서 루시퍼라제 및 녹색 형광 단백질 발현을 수반하는 비침습적 모니터링 방법을 설명한다. 이 프로토콜은 마우스에서 실시간으로 종양 형성 및 전이성 콜로니화를 모니터링하는 기술을 제공한다.
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