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DOI :
10.3791/63068-v
•
10:45 min
October 14th, 2021
Chapters
0:05
Introduction
1:11
Embryo Extraction
1:44
Ex Utero and In Utero Electroporation
3:35
Brain Extraction and Embedding in Agarose
4:39
Organotypic Slice Culture
5:43
Image Acquisition and Data Analysis
8:29
Results: In Situ Axon Growth and Growth Cone Dynamics
9:56
Conclusion
이 프로토콜은 현장 축삭 성장 및 성장 원뿔 역학 을 연구하는 간단하고 강력한 방법을 보여줍니다. 생체외 생리학적으로 관련된 급성 뇌 조각을 준비하는 방법을 설명하고 사용자 친화적인 분석 파이프라인을 제공합니다.
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