Deutsches Zentrum für Neurodegenerative Erkrankungen (DZNE)

1 ARTICLES PUBLISHED IN JoVE

Neuroscience

In Situ Visualization of Axon Growth and Growth Cone Dynamics in Acute Ex Vivo Embryonic Brain Slice Cultures

Eissa Alfadil¹, Frank Bradke¹, Sebastian Dupraz¹

¹Laboratory of Axon Growth and Regeneration, Deutsches Zentrum für Neurodegenerative Erkrankungen (DZNE)

This protocol demonstrates a straightforward and robust method to study in situ axon growth and growth cone dynamics. It describes how to prepare ex vivo physiologically relevant acute brain slices and provides a user-friendly analysis pipeline.