Coleta, Expansão e Diferenciação de Modelos Primários de Células Epiteliais Nasais Humanas para Quantificação da Frequência de Batimento de Cílios
November 10th, 2021
•Katelin M. Allan1,2, Sharon L. Wong1,2, Laura K. Fawcett1,2,3, Alexander Capraro1,2, Adam Jaffe1,2,3, Cristan Herbert4, Elvis Pandzic5, Shafagh A. Waters1,2,3
1School of Women's and Children's Health, Faculty of Medicine and Health, University of New South Wales, 2Molecular and Integrative Cystic Fibrosis Research Centre (miCF RC), Faculty of Medicine and Health, University of New South Wales, 3Department of Respiratory Medicine, Sydney Children's Hospital, 4Department of Pathology, School of Medical Sciences, University of New South Wales Australia, 5Katharina Gaus Light Microscopy Facility, Mark Wainwright Analytical Centre, University of New South Wales
Este protocolo descreve a coleta, expansão e diferenciação de células epiteliais nasais para modelos de células epiteliais organotípicas das vias aéreas e quantificação da frequência de batimentos de cílios por meio de imagens de células vivas e scripts personalizados.
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved