Name: isolation of epithelial cells from human dental follicle
Uploaded: 2021-11-05T13:00:00
Description: Watch this Scientific Journal Video about Isolation of Epithelial Cells from Human Dental Follicle at JoVE.com

这项研究得到了韩国国家研究基金会（NRF）的资助，该基金会由韩国政府资助（NRF-2017R1C1B2008406和NRF-2021R1F1A1064350）。Hee-Yeon Bae博士为初级培养提供了DF。

本研究已获江东庆熙大学医院机构审查委员会批准（IRB批准号：KHNMC 2017-06-009）。
1. 收集DF
注意：患者在手术前给予知情同意，以切除成熟或未成熟的受影响的第三磨牙。排除了患有以下疾病的患者：糖尿病，高血压，肺结核，肝炎，获得性免疫缺陷综合征。孕妇也被排除在外。
<ol><li>在手术提取受影响的第三磨牙期间收获DF（<strong class="xf...

<ol>
	<li>Tucker, A., Sharpe, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+cutting-edge+of+mammalian+development;+How+the+embryo+makes+teeth.">The cutting-edge of mammalian development; How the embryo makes teeth.</a> Nature Reviews Genetics. 5 (7), 499-508 (2004).</li><li>Choung, H. 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C., Chai, Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Fate+of+HERS+during+tooth+root+development.">Fate of HERS during tooth root development.</a> Developmental Biology. 334 (1), 22-30 (2009).</li><li>Farea, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Isolation+and+enhancement+of+a+homogenous+in+vitro+human+Hertwig's+epithelial+root+sheath+cell+population.">Isolation and enhancement of a homogenous in vitro human Hertwig's epithelial root sheath cell population.</a> International Journal of Molecular Sciences. 14 (6), 11157-11170 (2013).</li><li>Jung, H. S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Directing+the+differentiation+of+human+dental+follicle+cells+into+cementoblasts+and/or+osteoblasts+by+a+combination+of+HERS+and+pulp+cells.">Directing the differentiation of human dental follicle cells into cementoblasts and/or osteoblasts by a combination of HERS and pulp cells.</a> Journal of Molecular Histology. 42 (3), 227-235 (2011).</li><li>Fang, J., Tang, L., Liu, X. H., Wen, L. Y., Jin, Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Changes+of+the+unique+odontogenic+properties+of+rat+apical+bud+cells+under+the+developing+apical+complex+microenvironment.">Changes of the unique odontogenic properties of rat apical bud cells under the developing apical complex microenvironment.</a> International Journal of Oral Science. 1 (1), 26-33 (2009).</li><li>Oh, J. E., Yi, J. K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Isolation+and+characterization+of+dental+follicle-derived+Hertwig's+epithelial+root+sheath+cells.">Isolation and characterization of dental follicle-derived Hertwig's epithelial root sheath cells.</a> Clinical Oral Investigations. 25 (4), 1787-1796 (2021).</li><li>Oh, J. E., Kim, R. H., Shin, K. H., Park, N. H., Kang, M. K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Delta+Np63+alpha+protein+triggers+epithelial-mesenchymal+transition+and+confers+stem+cell+properties+in+normal+human+keratinocytes.">Delta Np63 alpha protein triggers epithelial-mesenchymal transition and confers stem cell properties in normal human keratinocytes.</a> Journal of Biological Chemistry. 286 (44), 38757-38767 (2011).</li><li>Morsczeck, C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Isolation+of+precursor+cells+(PCs)+from+human+dental+follicle+of+wisdom+teeth.">Isolation of precursor cells (PCs) from human dental follicle of wisdom teeth.</a> Matrix Biology. 24 (2), 155-165 (2005).</li><li>Nam, H., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Expression+profile+of+the+stem+cell+markers+in+human+Hertwig's+epithelial+root+sheath/Epithelial+rests+of+Malassez+cells.">Expression profile of the stem cell markers in human Hertwig's epithelial root sheath/Epithelial rests of Malassez cells.</a> Molecular and Cells. 31 (4), 355-360 (2011).</li><li>Lee, J. H., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Dental+follicle+cells+and+cementoblasts+induce+apoptosis+of+ameloblast-lineage+and+Hertwig's+epithelial+root+sheath/epithelial+rests+of+Malassez+cells+through+the+Fas-Fas+ligand+pathway.">Dental follicle cells and cementoblasts induce apoptosis of ameloblast-lineage and Hertwig's epithelial root sheath/epithelial rests of Malassez cells through the Fas-Fas ligand pathway.</a> European Journal of Oral Sciences. 120 (1), 29-37 (2012).</li><li>Wan, A. C. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Recapitulating+cell-cell+Interactions+for+organoid+construction+-+are+biomaterials+dispensable.">Recapitulating cell-cell Interactions for organoid construction - are biomaterials dispensable.</a> Trends in Biotechnology. 34 (9), 711-721 (2016).</li><li>Guo, Y., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Are+Hertwig's+epithelial+root+sheath+cells+necessary+for+periodontal+formation+by+dental+follicle+cells.">Are Hertwig's epithelial root sheath cells necessary for periodontal formation by dental follicle cells.</a> Archives of Oral Biology. 94, 1-9 (2018).</li><li>Sugaya, T., Tomita, M., Motoki, Y., Miyaji, H., Kawamami, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Influence+of+enamel+matrix+derivative+on+healing+of+root+surfaces+after+bonding+treatment+and+intentional+replantation+of+vertically+fractured+roots.">Influence of enamel matrix derivative on healing of root surfaces after bonding treatment and intentional replantation of vertically fractured roots.</a> Dental Traumatology. 32 (5), 397-401 (2016).</li><li>Fong, H. K., Foster, B. L., Popowics, T. E., Somerman, M. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+crowning+achievement:+getting+to+the+root+of+the+problem.">The crowning achievement: getting to the root of the problem.</a> Journal of Dental Education. 69 (5), 555-570 (2005).</li><li>Bonczek, O., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Tooth+agenesis:+What+do+we+know+and+is+there+a+connection+to+cancer.">Tooth agenesis: What do we know and is there a connection to cancer.</a> Clinical Genetics. 99 (4), 493-502 (2021).</li></ol>

此协议包括关键步骤。收获单细胞群对于从DF中成功分离上皮细胞至关重要。我们试图根据我们的假设从DF中分离出上皮细胞，即DF中有更多的上皮细胞。切碎程序增强了细胞从DF的分离和释放。切碎过程得到改善，并重复切碎，直到DF出现浆状，以促进单细胞的释放。获得最大数量的单细胞增加了上皮群体出现的可能性。在从DF中分离上皮细胞期间经常发生污染。使用40μm过滤器可最大限度地减少?...

牙齿形成始于口腔上皮的内陷1。根据牙齿发育阶段，口腔上皮有不同的名称，包括内外珐琅质上皮，宫颈袢和赫特维格上皮根鞘（HERS）。上皮区室与周围的间充质细胞相通。上皮-间充质相互作用调节牙齿形成和组织再生。获取牙齿上皮细胞，如口腔角质形成细胞和赫特维格上皮根鞘细胞（HERSC），对于研究牙齿上皮-间充质相互作用至关重要2。
啮齿动物来源的牙齿上皮细胞从上皮结构中分离出来，例如HERS。Li及其同事在收获了8日龄大鼠发育中的牙齿胚芽的顶端部分后，分离并永生化了大鼠摩尔衍生的HERSC3。HERS在放大镜下从顶端组织分离出来。考虑到牙齿发育阶段和年龄，由于伦理问题，从人类身上收获HERS几乎是不可能的：需要从幼儿身上去除发育中的牙齿胚芽才能收获人类的HERS。未成熟的牙齿细菌很少被提取。人牙齿上皮细胞可以从牙龈和牙周韧带（PDL）中分离出来。上皮结构衍生的细胞与间充质成分一起参与牙齿的形成，并且可能比口腔角质形成细胞更适合研究牙齿上皮 - 间充质相互作用。Malassez（ERM）的上皮细胞残留物是HERS衍生的上皮残留物，并且少量存在于PDL4中。研究报告了人类HERSC与PDL5的分离。然而，从PDL组织中收获人类HERSC并不总是成功的，因为该位置上皮种群的稀缺性5，6。
虽然啮齿类动物来源的 HERSC 维持在含血清的培养基中3，7，但人 DF 来源的上皮细胞是用类似于其他人上皮细胞的无血清培养基培养的，例如正常人表皮角质形成细胞和正常人口腔角质形成细胞8，9。这意味着啮齿动物牙齿上皮细胞和人牙齿上皮细胞之间的生理或功能差异。了解牙齿上皮 - 间充质相互作用的机制可能有助于临床应用的发展，包括再植期间的牙周重新附着，牙周病中的牙周再生，牙髓 - 牙本质复合物再生和生物牙齿生成。考虑到转化研究的特点，人牙齿上皮细胞可能比啮齿动物牙齿上皮细胞更适合研究上皮-间充质相互作用。
人类DF是松散的结缔组织，通常位于受累的牙齿中。DF含有间充质前体10。然而，据我们所知，没有研究报告在2021年之前从牙囊中分离出上皮细胞。Oh和Yi在20218年报告了从人类DF中分离出上皮细胞的情况。通过蛋白质印迹和形态学分析证实上皮表型。对DF衍生上皮细胞起源的分析显示出与其他研究相似的结果。DF来源的上皮细胞既不是内皮细胞也不是造血细胞5，11，Oh和Yi建议将这些细胞命名为DF-HERSC。DF的体积大于PDL，并且可以从DF中分离出更多的上皮细胞。这增强了上皮菌落的出现，并导致从DF中收获上皮细胞的成功率很高。这项研究建议使用DF作为分离牙齿上皮细胞的组织源。
在本研究中，根据先前描述的程序从DF中分离出单个细胞10，12。DF包含异质性细胞群，并且在手术的早期阶段可能存在几种细胞类型。Morsczek及其同事分离出DF衍生的间充质干细胞10。我们假设DF含有上皮细胞，只有上皮细胞才能在无血清条件下存活。这项研究与Morsczek等人在上皮群体的选择和间充质细胞的抑制方面有所不同。选择使用角质形成细胞血清无培养基（SFM）进行，其允许上皮细胞增殖并抑制间充质细胞增殖。这项研究起源于Oh和Yi8的一份报告。本研究的目的是描述该报告中使用的从人DF中分离上皮细胞的方法的细节。

<table border="1" fo:keep-together.within-page="1" fo:keep-with-next.within-page="always">
	<tbody>
		<tr>
			<td>EMSURE ACS,ISO,Reag. Ph Eur 2-Propanol</td>
			<td>EMD millipore Co., MA, USA</td>
			<td>1096341011</td>
			<td>1 L</td>
		</tr>
		<tr>
			<td>0.05% trypsin-EDTA</td>
			<td>Gibco, Grand island, NY, USA</td>
			<td>25300054</td>
			<td>100 mL</td>
		</tr>
		<tr>
			<td>40 &#956;m cell strainer</td>
			<td>Falcon, NC, USA</td>
			<td>352340</td>
			<td></td>
		</tr>
		<tr>
			<td>Cell culture dish (100 x 20 mm)</td>
			<td>Thermo Fisher Scientific Inc., Waltham, USA</td>
			<td>172958</td>
			<td>Discontinued</td>
		</tr>
		<tr>
			<td>Cell culture dish (60 x 15 mm)</td>
			<td>Thermo Fisher Scientific Inc., Waltham, USA</td>
			<td>150326</td>
			<td></td>
		</tr>
		<tr>
			<td>Collagenase type 1</td>
			<td>Gibco, Grand island, NY, USA</td>
			<td>17100017</td>
			<td>1 g</td>
		</tr>
		<tr>
			<td>Combi-514R / Refrigerated large capacity centrifuge</td>
			<td>Hanil science industrial Co., LTD., Daejeon, South Korea</td>
			<td>CB-514R</td>
			<td></td>
		</tr>
		<tr>
			<td>Conical tube</td>
			<td>SPL Life Sciences Co., Ltd., Gyeonggi-do, South Korea</td>
			<td>50015 
			50050</td>
			<td>15 mL 
			50 mL</td>
		</tr>
		<tr>
			<td>Cryogenic vial</td>
			<td>Corning Inc., NY, US</td>
			<td>430488</td>
			<td>2 mL</td>
		</tr>
		<tr>
			<td>Dimethyl sulfoxide (DMSO)</td>
			<td>Sigma-Aldrich, Missouri, US</td>
			<td>D2650-100ml</td>
			<td>100 mL</td>
		</tr>
		<tr>
			<td>Dispase</td>
			<td>Gibco, Grand island, NY, USA</td>
			<td>17105041</td>
			<td>1 g</td>
		</tr>
		<tr>
			<td>Dulbecco&#39;s Phosphate-Buffered Saline</td>
			<td>Welgene Inc., Gyengsangbuk-do, South Korea</td>
			<td>&#160;LB 001-02</td>
			<td>500 mL</td>
		</tr>
		<tr>
			<td>Fetal bovine serum</td>
			<td>Gibco, Grand island, NY, USA</td>
			<td>16000044</td>
			<td>500 mL</td>
		</tr>
		<tr>
			<td>Heraeus BB 15 / CO2 incubator</td>
			<td>Thermo Fisher Scientific Inc., Waltham, USA</td>
			<td>51023121</td>
			<td></td>
		</tr>
		<tr>
			<td>Keratinocyte serum-free medium</td>
			<td>Gibco, Grand island, NY, USA</td>
			<td>10724-011</td>
			<td>500 mL</td>
		</tr>
		<tr>
			<td>MVE CryoSystem 2000</td>
			<td>MVE Biological Solutions Co., GA, USA</td>
			<td>CryoSystem 2000</td>
			<td></td>
		</tr>
		<tr>
			<td>Nalgene Mr. Frosty Freezing Container</td>
			<td>Thermo Fisher Scientific Inc., Waltham, USA</td>
			<td>5100-0001</td>
			<td>for 1.2-2 mL CryoVials</td>
		</tr>
		<tr>
			<td>Olympus CKX41 / Inverted cell culture microscope</td>
			<td>Olympus Life Science, 
			Waltham, Massachusetts</td>
			<td>22-00723-01</td>
			<td>Discontinued</td>
		</tr>
		<tr>
			<td>Penicillin-Streptomycin Strep</td>
			<td>Gibco, Grand island, NY, USA</td>
			<td>15140122</td>
			<td>100 mL (10,000 U/mL)</td>
		</tr>
		<tr>
			<td>Pipet aid XP</td>
			<td>Drummond scientific Co., PA, USA</td>
			<td>HDR-4-000-201</td>
			<td></td>
		</tr>
		<tr>
			<td>Pipetman Classic P1000</td>
			<td>Gilson, Villiers le Bel, France</td>
			<td>F123602</td>
			<td>100-1000 &#181;L</td>
		</tr>
		<tr>
			<td>Refrigerant</td>
			<td>Nihon freezer Co. Ltd., Tokyo, Japan</td>
			<td>CLN 540U</td>
			<td>~-80 &#176;C / Discontinued</td>
		</tr>
		<tr>
			<td>Serological pipet</td>
			<td>SPL Life Sciences Co., Ltd., Gyeonggi-do, South Korea</td>
			<td>91010</td>
			<td>10ml</td>
		</tr>
		<tr>
			<td>TrypLE Express Enzyme (1x), phenol red</td>
			<td>Thermo Fisher Scientific Inc., Waltham, USA</td>
			<td>12605010</td>
			<td>cell dissociation protease</td>
		</tr>
	</tbody>
</table>

isolation of epithelial cells from human dental follicle

牙囊（DF）是在口腔颌面外科医生切除受影响的第三磨牙期间收获的。在DF收获当天进行上皮细胞分离。用DPBS洗涤DFBS三次，然后用组织剪刀解剖，直到组织具有浆状或粘稠度。通过离心造粒单细胞群并用角质形成细胞无血清培养基洗涤。异质细胞群分布在培养皿中。使用角质形成细胞血清无培养基选择上皮细胞。每天更换培养基，直到没有观察到漂浮的碎片或死细胞。上皮细胞在细胞群分布后7-10天内出现。上皮细胞在无血清培养基中存活，而α修饰的最小必需培养基补充10%胎牛血清允许间充质型细胞增殖。DF是分离牙齿上皮细胞的组织源。
本研究的目的是建立一种从人DF中分离上皮细胞的方法。牙周韧带（PDL）用于分离人牙齿上皮细胞。由于组织体积小，从人PDL获取上皮细胞并不总是成功的，导致上皮细胞数量少。DF的体积比PDL大，并且包含更多的细胞。DF可以是人牙齿上皮细胞原代培养的组织来源。该协议比使用PDL的隔离方法更容易，更有效。获取人类牙齿上皮细胞可能有助于进一步研究牙齿上皮 - 间充质相互作用。

脱模剂收获 手术由口腔颌面外科医生进行。由外科医生收集人源材料，包括牙齿碎片，牙龈组织和DF（图1A）。DF可能附着在牙齿碎片上。口腔颌面外科医生将能够识别DF。组织收集需要与外科医生的合作和沟通。DF是一种形状不规则的膜样组织。牙龈组织具有角质化表面，可与DF区分开来。牙髓组织驻留在牙髓室中，在手术过程中很少与牙齿分离。使用前将DF?...

Watch this Scientific Journal Video about Isolation of Epithelial Cells from Human Dental Follicle at JoVE.com

Isolation of Epithelial Cells from Human Dental Follicle

牙囊含有上皮群和间充质细胞。通过提供独特的培养基，从异质性牙卵泡细胞群中选择上皮群体。上皮细胞存活并在无血清培养基中形成菌落。

从人牙囊中分离上皮细胞

The dental follicle (DF) was harvested during the removal of an impacted third molar by an oral maxillofacial surgeon. Epithelial cell isolation was ...

该协议展示了一种用于获取人类牙齿上皮细胞的增强方法。在手术过程中很难识别聚集的细胞沉淀。处理上清液时要小心，以防止细胞群丢失。首先，在手术拔除受影响的第三磨牙期间收获牙囊。开始细胞分离程序或将牙科卵泡储存在4摄氏度的DPBS中，含3%青霉素 - 链霉素。要清洗牙囊，请用镊子握住牙囊并将其放入洗涤管1中。轻轻摇晃10至15次。取出并将其放入管2中。再次摇晃10到15次。最后，将其取出并放入管3中，然后摇动它。洗涤三次后，将牙囊置于60毫米培养皿中。用组织剪刀切碎组织，直到牙囊具有纸浆状或粘稠的外观。使用培养皿的一小段侧部，以尽量减少组织损失。将1型胶原酶和蛋白酶溶液各混合一毫升，放入15毫升锥形管中。将切碎的组织转移到15毫升的锥形管中，然后轻轻摇晃并在37摄氏度下孵育一小时。向试管中加入5毫升0.05%胰蛋白酶-EDTA。摇晃并在37摄氏度下孵育15分钟。制备含有角质形成细胞血清游离培养基和10%胎牛血清的角质形成细胞培养基。将三毫升角质形成细胞培养基加入新的50毫升锥形管中。将40微米的过滤器放在该管的顶部。接下来，用10毫升血清学移液管从含有组织的管中吸出上清液，并将其通过过滤器。将收集的悬浮液转移到15毫升的锥形管中。离心管并除去上清液。然后加入三毫升角质形成细胞血清游离培养基。再离心三分钟，取出上清液。使用无角质形成细胞血清培养基将单个细胞群平板到60毫米培养皿中。在37摄氏度的5%二氧化碳的加湿气氛中保持培养皿的最终体积为5毫升。具有上皮形态的细胞在接种单细胞群后7至10天内出现。鹅卵石形上皮细胞的数量在出现时从1到10不等，并且随着时间的推移细胞扩张成菌落。最关键的一步是将牙囊切碎成小颗粒。它增强了单个细胞与卵泡组织的分离。间充质细胞可以从人牙囊中分离出来。含有血清的培养环境选择间充质细胞并抑制单细胞群的上皮生长。该协议提供了获取人牙齿上皮细胞的方法。牙齿卵泡来源的上皮细胞可用于研究牙齿上皮 - 间充质相互作用。

Research

JoVE Journal

Biology

Primary Human Bronchial Epithelial Cells Grown from Explants

从外植体生长的首要人权支气管上皮细胞

Human bronchial epithelial cells are needed for cell models of disease and to investigate the effect of excipients and pharmacologic agents on the ...

科研

生物学

Isolation of Stem Cells from Human Pancreatic Cancer Xenografts

人胰腺癌移植瘤的干细胞的分离

Cancer stem cells (CSCs) have been identified in a growing number of malignancies and are functionally defined by their ability to undergo self-renewal ...

Isolation and Culture of Adult Epithelial Stem Cells from Human Skin

成人从人体皮肤的上皮干细胞的分离和培养

The homeostasis of all self-renewing tissues is dependent on adult stem cells. As undifferentiated stem cells undergo asymmetric divisions, they generate ...

Isolation of Mammary Epithelial Cells from Three-dimensional Mixed-cell Spheroid Co-culture

从三维混合细胞球体共培养的乳腺上皮细胞的隔离

While enormous efforts have gone into identifying signaling pathways and molecules involved in normal and malignant cell behaviors1-2, much of this work ...

Chromatin Isolation by RNA Purification (ChIRP)

Chromatin Isolation by RNA Purification ChIRP

RNA纯化的染色质隔离（CHIRP）

Long noncoding RNAs are key regulators of chromatin states for important biological processes such as dosage compensation, imprinting, and developmental ...

Na&iuml;ve Adult Stem Cells Isolation from Primary Human Fibroblast Cultures

Na&#239;ve Adult Stem Cells Isolation from Primary Human Fibroblast Cultures

天真成人干细胞的分离从初级人类成纤维细胞培养

Over the last decade, several adult stem cell  populations have been identified in human skin 1-4.  The isolation of multipotent adult dermal precursors ...

Induction and Analysis of Epithelial to Mesenchymal Transition

诱导上皮和分析间质转化

Epithelial  to mesenchymal transition (EMT) is essential for proper morphogenesis during  development. Misregulation of this  process has been implicated ...

Manual Isolation of Adipose-derived Stem Cells from Human Lipoaspirates

从人类Lipoaspirates脂肪来源干细胞的手动隔离

In 2001, researchers at the University of  California, Los Angeles, described the isolation of a new population of adult  stem cells from liposuctioned ...

Isolation and Culture of Dental Epithelial Stem Cells from the Adult Mouse Incisor

从成年小鼠切牙牙源性上皮干细胞的分离和培养

Understanding the cellular and molecular mechanisms that underlie tooth regeneration and renewal has become a topic of great interest1-4, and the mouse ...

Isolation and Enrichment of Human Lung Epithelial Progenitor Cells for Organoid Culture

分离和富集人肺上皮祖细胞用于类器官培养

Epithelial organoid models serve as valuable tools to study the basic biology of an organ system and for disease modeling. When grown as organoids, ...