Name: a porcine heterotopic heart transplantation protocol for delivery of therapeutics to a cardiac allograft
Uploaded: 2022-02-14T14:30:00
Description: Watch this Scientific Journal Video about A Porcine Heterotopic Heart Transplantation Protocol for Delivery of Therapeutics to a Cardiac Allograft at JoVE.com

我们要感谢杜克大学大型动物外科核心和杜克大学灌注服务公司在这些手术过程中的帮助。我们还要感谢保罗·莱兹伯格和跨媒体公司的支持。

注意：选择两头雌性尤卡坦猪，其中一头被指定为心脏移植供体，另一头被指定为受体。建议6-8个月大的猪，体重约30公斤，血型相容。该协议的概述如图 1所示。猪的住房和处理程序是根据杜克大学医学中心动物护理和使用委员会的指导方针进行的。
1. 离体灌注装置的制备
<ol><li>准备 离体 灌注装置和细胞保护装置，以便根?...

<ol>
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L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Successful+heart+transplant+after+ten+hours+out-of-body+time+using+the+TransMedics+Organ+Care+System.">Successful heart transplant after ten hours out-of-body time using the TransMedics Organ Care System.</a> Heart, Lung &amp; Circulation. 24 (6), 611-613 (2015).</li><li>Ragalie, W. S., Ardehali, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Current+status+of+normothermic+ex-vivo+perfusion+of+cardiac+allografts.">Current status of normothermic ex-vivo perfusion of cardiac allografts.</a> Current Opinion in Organ Transplantation. 25 (3), 237-240 (2020).</li><li>Koerner, M. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Normothermic+ex+vivo+allograft+blood+perfusion+in+clinical+heart+transplantation.">Normothermic ex vivo allograft blood perfusion in clinical heart transplantation.</a> Heart Surgery Forum. 17 (3), 141-145 (2014).</li><li>Rosenbaum, D. H., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Perfusion+preservation+versus+static+preservation+for+cardiac+transplantation:+effects+on+myocardial+function+and+metabolism.">Perfusion preservation versus static preservation for cardiac transplantation: effects on myocardial function and metabolism.</a> Journal of Heart and Lung Transplantation. 27 (1), 93-99 (2008).</li><li>Cullen, P. P., Tsui, S. S., Caplice, N. M., Hinchion, J. 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K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Gene+therapy+for+cardiovascular+disease:+advances+in+vector+development,+targeting,+and+delivery+for+clinical+translation.">Gene therapy for cardiovascular disease: advances in vector development, targeting, and delivery for clinical translation.</a> Cardiovascular Research. 108 (1), 4-20 (2015).</li><li>Kieserman, J. M., Myers, V. D., Dubey, P., Cheung, J. Y., Feldman, A. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Current+landscape+of+heart+failure+gene+therapy.">Current landscape of heart failure gene therapy.</a> Journal of the American Heart Association. 8 (10), 012239 (2019).</li><li>Perin, E. C., Perin, E. C., Miller, L. W., Taylor, D. A., Wilkerson, J. T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> Stem cell and gene therapy for cardiovascular disease. , 279-287 (2016).</li><li>Bishawi, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+normothermic+ex+vivo+organ+perfusion+delivery+method+for+cardiac+transplantation+gene+therapy.">A normothermic ex vivo organ perfusion delivery method for cardiac transplantation gene therapy.</a> Scientific Reports. 9 (1), 8029 (2019).</li><li>Hulot, J. S., Ishikawa, K., Hajjar, R. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Gene+therapy+for+the+treatment+of+heart+failure:+promise+postponed.">Gene therapy for the treatment of heart failure: promise postponed.</a> European Heart Journal. 37 (21), 1651-1658 (2016).</li><li>Ho, C. S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Molecular+characterization+of+swine+leucocyte+antigen+class+I+genes+in+outbred+pig+populations.">Molecular characterization of swine leucocyte antigen class I genes in outbred pig populations.</a> Animal Genetics. 40 (4), 468-478 (2009).</li><li>Ho, C. S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Molecular+characterization+of+swine+leucocyte+antigen+class+II+genes+in+outbred+pig+populations.">Molecular characterization of swine leucocyte antigen class II genes in outbred pig populations.</a> Animal Genetics. 41 (4), 428-432 (2010).</li></ol>

在心脏移植中离体灌注期间提供治疗药物提供了一种改变同种异体移植物并可能改善移植结果的策略。这里介绍的方案结合了最先进的正常体温离体血灌注储存，并为测试细胞，基因或免疫疗法向同种异体移植物11，12，13的分离递送提供了有希望的潜力。迄今为止，这些心血管疾病和终末期心力衰竭推定疗法的心...

心力衰竭是一种影响美国约600万成年人的疾病，预计到2030年将增加到800万成年人1。心脏移植是终末期心力衰竭的黄金标准治疗。但是，它并非没有局限性和复杂性。它仍然受到可用供体心脏数量，原发性移植物功能障碍，心脏排斥反应以及长期免疫抑制的副作用的限制 2.这些限制在可能经历同种异体移植失败并需要随后重新移植以达到正常预期寿命的年轻受者中尤为重要。
克服这些限制的理想干预措施是在植入受体之前用治疗方法治疗整个心脏同种异体移植物，这可以提高同种异体移植物的生存能力并赋予“心脏保护”。这种干预将预防性地进行，以尽量减少缺血性损伤、同种异体移植物排斥反应、心脏同种异体移植血管病变甚至修复边缘同种异体移植物的发生率。开发这些类型的干预措施的转化研究需要心脏移植的大型动物模型，以便对心脏移植物进行长期监测。位于腹内位置的猪异位心脏移植模型已被证明是实现这一目的的理想选择。心脏移植在这个位置允许测试新疗法的效果，并评估移植物排斥反应的免疫病理学。此外，异位模型优于原位模型，因为受者的总生存率更高，不需要体外循环，也不需要移植物来维持受体的循环3.
向心脏有效提供治疗性干预措施，如基因、细胞或免疫疗法，是临床应用的重要障碍4，5。 离体 灌注装置引入的技术允许移植物持续灌注，使其保持在非工作但代谢活性状态6，7，8，9。这提供了一个独特的机会，用先进的治疗方法治疗整个心脏，同时最大限度地减少全身递送的潜在副作用10，11，12，13。利用 离体 灌注装置进行治疗性递送的另一个优点是，它们允许在较长的时间内向冠状动脉循环施用药物，而使用传统的冷静态储存方法则不可行。这允许将疗法更多地全球递送到移植物14。使用这里介绍的方案，我们成功地使用腺病毒载体15将萤火虫荧光素酶基因递送到整个猪心脏移植物中。该方案的目的是提供一种可重复且稳健的方法，用于在移植前将治疗性地递送到整个心脏同种异体移植物。

<table border="1" fo:keep-together.within-page="1" fo:keep-with-next.within-page="always">
	<tbody>
		<tr>
			<td>0 Looped Maxon suture</td>
			<td>Covidien</td>
			<td>GMM-341L</td>
			<td>Used to close fascia of the laparotomy incision</td>
		</tr>
		<tr>
			<td>0 Silk ties</td>
			<td>Medtronic, Inc</td>
			<td>S346</td>
			<td></td>
		</tr>
		<tr>
			<td>18 G Angiocath</td>
			<td>BD</td>
			<td>381144</td>
			<td>Used to de-air the left ventricle of the donor heart after implantation</td>
		</tr>
		<tr>
			<td>20 Fr LV vent</td>
			<td>Medtronic, Inc</td>
			<td>12002</td>
			<td></td>
		</tr>
		<tr>
			<td>2-0 Silk sutures</td>
			<td>Ethicon, Inc.</td>
			<td>SA11G</td>
			<td></td>
		</tr>
		<tr>
			<td>2-0 Silk ties</td>
			<td>Ethicon, Inc.</td>
			<td>SA65H</td>
			<td></td>
		</tr>
		<tr>
			<td>2-0 Vicryl suture</td>
			<td>Ethicon, Inc.</td>
			<td>J259H</td>
			<td></td>
		</tr>
		<tr>
			<td>24 Fr venous cannula</td>
			<td>Medtronic, Inc</td>
			<td>68124</td>
			<td></td>
		</tr>
		<tr>
			<td>3-0 Prolene sutures</td>
			<td>Ethicon, Inc.</td>
			<td>8522</td>
			<td></td>
		</tr>
		<tr>
			<td>4-0 Monocryl suture</td>
			<td>Ethicon, Inc.</td>
			<td>Y469G</td>
			<td></td>
		</tr>
		<tr>
			<td>4-0 Prolene sutures</td>
			<td>Ethicon, Inc.</td>
			<td>8521</td>
			<td></td>
		</tr>
		<tr>
			<td>Animal hair cutting clipper</td>
			<td>Wahl</td>
			<td>8786-452</td>
			<td></td>
		</tr>
		<tr>
			<td>Aortic clamp</td>
			<td>V. Mueller</td>
			<td>CH6201</td>
			<td></td>
		</tr>
		<tr>
			<td>Army Navy retractor</td>
			<td>V. Mueller</td>
			<td>SU3660</td>
			<td></td>
		</tr>
		<tr>
			<td>ATF 40, Cell saver disposable set</td>
			<td>Fresenius Kabi</td>
			<td>9108494</td>
			<td>Cell saver device insert</td>
		</tr>
		<tr>
			<td>Balfour retractor</td>
			<td>V. Mueller</td>
			<td>SU3042</td>
			<td>Used as an abdominal wall retractor</td>
		</tr>
		<tr>
			<td>C.A.T.S cell saver</td>
			<td>Fresenius Kabi</td>
			<td>ES0019</td>
			<td>Cell saver device used to wash donor blood</td>
		</tr>
		<tr>
			<td>Cardiac defibrillator</td>
			<td>Zoll</td>
			<td>M Series</td>
			<td>Cardiac defibrillator</td>
		</tr>
		<tr>
			<td>Castro needle holder</td>
			<td>V. Mueller</td>
			<td>CH8589</td>
			<td></td>
		</tr>
		<tr>
			<td>CG4 iStat cartridges</td>
			<td>Abbott</td>
			<td>03P85-25</td>
			<td>POC testing</td>
		</tr>
		<tr>
			<td>CG8 iStat cartridges</td>
			<td>Abbott</td>
			<td>03P88-25</td>
			<td>POC testing</td>
		</tr>
		<tr>
			<td>DeBakey forceps</td>
			<td>V. Mueller</td>
			<td>CH5902</td>
			<td></td>
		</tr>
		<tr>
			<td>Electrocautery disposable pencil</td>
			<td>Covidien</td>
			<td>E2450H</td>
			<td></td>
		</tr>
		<tr>
			<td>Gerald forceps</td>
			<td>V. Mueller</td>
			<td>NL1451</td>
			<td></td>
		</tr>
		<tr>
			<td>Hemotherm 400CE Dual Reservoir Cooler/Heater</td>
			<td>Cincinnati Sub-Zero</td>
			<td>86022</td>
			<td>Heater cooler used to regulate perfusion temperature on the ex vivo perfusion device</td>
		</tr>
		<tr>
			<td>iSTAT 1</td>
			<td>Abbott</td>
			<td>04P75-03</td>
			<td>POC testing device</td>
		</tr>
		<tr>
			<td>Kocher clamp</td>
			<td>V. Mueller</td>
			<td>SU2790</td>
			<td></td>
		</tr>
		<tr>
			<td>Large clip applier</td>
			<td>Sklar</td>
			<td>50-4300</td>
			<td></td>
		</tr>
		<tr>
			<td>Large clips</td>
			<td>Teleflex</td>
			<td>4200</td>
			<td></td>
		</tr>
		<tr>
			<td>Large soft pledgets</td>
			<td>Covidien</td>
			<td>8886867901</td>
			<td></td>
		</tr>
		<tr>
			<td>Medium clip applier</td>
			<td>Sklar</td>
			<td>50-4335</td>
			<td></td>
		</tr>
		<tr>
			<td>Medium clips</td>
			<td>Teleflex</td>
			<td>2200</td>
			<td></td>
		</tr>
		<tr>
			<td>Metzenbaum scissor</td>
			<td>V. Mueller</td>
			<td>CH2006-001</td>
			<td></td>
		</tr>
		<tr>
			<td>No. 10 scalpel blade</td>
			<td>Swann-Mortan</td>
			<td>301</td>
			<td>Used for skin incision</td>
		</tr>
		<tr>
			<td>No. 11 scalpel blade</td>
			<td>Kiato Plus</td>
			<td>18111</td>
			<td>Used for vascular incision</td>
		</tr>
		<tr>
			<td>OCS device with base</td>
			<td>TransMedics, Inc.</td>
			<td></td>
			<td>Ex vivo perfusion device</td>
		</tr>
		<tr>
			<td>OCS disposable</td>
			<td>TransMedics, Inc.</td>
			<td></td>
			<td>Ex vivo perfusion device insert with perfusion kits</td>
		</tr>
		<tr>
			<td>Pacing cable</td>
			<td>Remington Medical</td>
			<td>FL-601-97</td>
			<td></td>
		</tr>
		<tr>
			<td>Pediatric cardioplegia catheter (4Fr)</td>
			<td>Medtronic, Inc</td>
			<td>10218</td>
			<td>Used to deliver cardioplegia to the donor aortic root</td>
		</tr>
		<tr>
			<td>Pediatric Foley catheter</td>
			<td>Teleflex</td>
			<td>RSH170003080</td>
			<td>Placed pre-op to decompress the recipient&#39;s bladder</td>
		</tr>
		<tr>
			<td>Potts scissors</td>
			<td>V. Mueller</td>
			<td>CH13038</td>
			<td></td>
		</tr>
		<tr>
			<td>Pressure bag x2 (1,000 mL)</td>
			<td>Novaplus</td>
			<td>V4010H</td>
			<td>Used to deliver cardioplegia at a set pressure</td>
		</tr>
		<tr>
			<td>Satinsky clamp</td>
			<td>V. Mueller</td>
			<td>CH7305</td>
			<td>Vascular clamp used for creating anastomoses between donor heart and recipient vessels</td>
		</tr>
		<tr>
			<td>Scissors</td>
			<td>Felco</td>
			<td>FELCO 200A-50</td>
			<td>Used to perform sternotomy</td>
		</tr>
		<tr>
			<td>Small hard pledgets</td>
			<td>Covidien</td>
			<td>8886867701</td>
			<td></td>
		</tr>
		<tr>
			<td>Sternal retractor</td>
			<td>V. Mueller</td>
			<td>CH6950-007</td>
			<td></td>
		</tr>
		<tr>
			<td>Temporary cardiac pacing wires</td>
			<td>Ethicon, Inc.</td>
			<td>TPW32</td>
			<td></td>
		</tr>
		<tr>
			<td>Temporary dual chamber pacemaker</td>
			<td>Medtronic, Inc</td>
			<td>5388</td>
			<td>Cardiac pacing device</td>
		</tr>
		<tr>
			<td>Tourniquet kit</td>
			<td>Medtronic, Inc</td>
			<td>79005</td>
			<td>Rummel tourniquets</td>
		</tr>
		<tr>
			<td>Umbilical tape</td>
			<td>Covidien</td>
			<td>8886861903</td>
			<td></td>
		</tr>
		<tr>
			<td>Vessel loops</td>
			<td>Covidien</td>
			<td>31145686</td>
			<td></td>
		</tr>
	</tbody>
</table>

a porcine heterotopic heart transplantation protocol for delivery of therapeutics to a cardiac allograft

心脏移植是终末期心力衰竭的黄金标准治疗。然而，它仍然受到可用供体心脏数量和并发症（如原发性移植物功能障碍和移植物排斥反应）的限制。最近在心脏移植中临床上使用 离体 灌注装置为通过治疗干预治疗心脏同种异体移植物提供了独特的机会，以改善功能并避免有害的受体反应。建立一个转化的大动物模型，用于治疗性地递送到整个同种异体移植物，对于测试心脏移植中的新治疗方法至关重要。腹内位置的猪异位心脏移植模型是评估新干预措施效果和移植物排斥反应免疫病理学的优秀模型。该模型还为猪提供了长期存活，因为不需要移植物来维持受体的循环。该方案的目的是提供一种可重复和稳健的方法，用于在移植前实现对整个心脏同种异体移植物的治疗 性离体 递送，并提供技术细节以对 离体 灌注的心脏进行生存异位移植。

根据研究设计，该小组在本文介绍的方案后5至35天内成功地存活了9头猪。在接受该方案的10头猪中，只有1头因手术并发症过早死亡，存活率为90%。 图2 所示为在猪腹内位置移植的异位心脏的配置图。在确定同种异体移植物的吻合部位时，选择一个可以最大限度地减少吻合上任何紧张或扭结的部位。这确保了吻合口正确愈合，并且同种异体移植物得到最佳的血液灌注和引流。...

Watch this Scientific Journal Video about A Porcine Heterotopic Heart Transplantation Protocol for Delivery of Therapeutics to a Cardiac Allograft at JoVE.com

A Porcine Heterotopic Heart Transplantation Protocol for Delivery of Therapeutics to a Cardiac Allograft

我们提出了一种方案，用于利用正常温离 体 血灌注系统在猪异位心脏移植模型中将治疗药物递送到整个心脏同种异体移植物。

一种猪异位心脏移植方案，用于向心脏同种异体移植物提供治疗药物

Cardiac transplantation is the gold standard treatment for end-stage heart failure. However, it remains limited by the number of available donor hearts ...

该协议首次描述了如何在心脏移植的大型动物模型中有效地将疗法施用于整个心脏同种异体移植物。该协议的主要优点是，它允许研究人员在移植后很长一段时间内测试治疗对整个心脏同种异体移植物的影响。该技术可用于研究预防心脏同种异体移植物排斥反应，原发性移植物功能障碍，最大限度地减少术后免疫抑制需求或延长心脏同种异体移植物寿命的干预措施。优化暴露是移植过程中最具挑战性的部分，需要多个团队成员才能完成。同样，需要有经验的灌注师来协调和监测离体灌注。首先，根据猪的大小，使用从牛皮向下到剑齿的数字10刀片做一个20到30厘米长的切口。使用电烙术将胸大肌从胸骨向下分开到剑突，小心地沿着胸骨的中线分开。一旦下降到胸骨，划中线，并通过用沉重的剪刀将其分开，从xiphoid开始胸骨切开术。然后在每次切口后延长胸骨切开术头颅。使用手指扫除将心脏与胸骨钝分开，并通过丘脑完成胸骨切开术。使用胸骨牵开器优化手术场暴露后，通过从横膈膜纵向进入心包到主动脉并使用丝线创建心包支架，用电烙术识别和切除胸腺。将组织完全分开在主动脉和肺动脉之间，并可视化主动脉弓和头侧躯干的位置，以便于正确放置主动脉交叉钳。使用剪刀和钝性解剖，环周释放上腔静脉，并在上腔静脉周围绑上两条零尺寸的丝线。将4-0号聚丙烯缝合线以U型缝合方式应用于上升主动脉，并以围网方式应用于右心房。插入由先前放置的U形缝线固定的小儿主动脉根套管。然后在将套管脱气后，用拉梅尔止血带固定到位。在主动脉根管被 del Nido 心脏麻痹冲洗后，将主动脉根插管连接到心脏麻痹管。在先前放置的钱包内创建右心房切开术。将静脉插管插入右心房，并用拉梅尔止血带固定。将静脉插管连接到连接到细胞储蓄器心脏切开术的无菌吸入管路，并收集约1至1.3升血液。然后应用主动脉交叉夹，小心地确保夹子完全遮挡上升的主动脉。在心脏上放置无菌冰泥后，将下腔静脉和上腔静脉分开，正好位于合子静脉的近端。弓水平端的主动脉刚好远端于无名动脉，主肺动脉位于分叉处。识别并连接肺静脉，然后从胸部取出心脏并将其放入装有无菌冰的容器中。为了准备主动脉以放置主动脉连接器，将四条承诺的4-0号聚丙烯缝合线以简单的水平床垫方式放置在远端主动脉内侧周围，在切割边缘以下五毫米处，然后将其绑住。在举起承诺的主动脉缝合线时，将主动脉连接器插入主动脉，并在主动脉周围系上脐带以固定连接器。在主肺动脉的远端切口边缘周围放置一根 4-0 号聚丙烯钱包。插入肺动脉插管并系上钱包的两端以固定套管。然后将肺动脉插管连接到设备上的肺动脉连接器，并用领带固定。将准备好的移植物从后台带到离体灌注装置，并将主动脉连接器连接到装置位置。左心室通气通过未系带的肺静脉流入左心房，穿过二尖瓣进入左心室。使用数字10刀片，做一个20至30厘米长的皮肤切口，然后使用电烙术继续解剖到筋膜。用两个Kocher钳抬起筋膜和腹膜后，使用Metzenbaum剪刀小心地在腹膜腔中做一个小切口。使用电烙术将腹膜开口延长至切口的整个长度。将手指放在下面以保护下面的内脏。放置 Balfour 牵开器以优化暴露，并使用湿毛巾颅内缩回小肠。将夹层向下带到下腔静脉的腹主动脉，然后用中大夹子连接淋巴管。在后台上，当左心室室通口插入的肺静脉左心房切开术被过度播种时，修剪主动脉和肺动脉的远端，其中连接到套管可能已经压碎了组织。将Satinsky夹在下腔静脉上，并使用数字11刀片和Pott剪刀创建约1.5厘米的纵向静脉切开术。使用运行尺寸为 4-0 的聚丙烯缝合线，以端对侧的方式将肺动脉移植物吻合到受者的肾下下腔静脉。然后使用运行尺寸为4-0的聚丙烯缝合线以末端到侧面的方式将主动脉移植物吻合到受体的肾下主动脉。小心地将心脏置于右侧腹膜后间隙，防止吻合口紧张和血管扭结。然后更换小肠。用环大小为零的Maxon缝合线以跑步方式关闭筋膜，从切口的两端开始，并在中间绑扎，避免对肠道造成任何伤害。以跑步方式关闭尺寸为2-0 Vicryl的真皮深层层，以跑步方式关闭尺寸为4-0单烯的真皮深层。获得了几种不同的灌注参数。从肺动脉、主动脉和冠状动脉测量循环流速。还测量了主动脉压力，包括平均压力，收缩压，舒张压。在离体灌注期间，还测量了心脏同种异体移植物的心率和温度。在灌注期间，从灌注液中测量混合静脉血氧饱和度和血细胞比容值。腹腔内异位心脏原位35天的存活率表明使用该方案移植成功。要记住的最重要的事情是为同种异体移植物选择一个位点，以最大限度地减少吻合口紧张和扭结的可能性。通过遵循这一程序，研究人员将能够测试几种不同类型的治疗方法，以确定它们对同种异体移植物的免疫反应的影响，并确定治疗作用对同种异体移植物的寿命。在开发该协议后，我们小组已经能够测试用于基因递送的不同病毒载体载体，以及急性同位异体排斥的模型，以了解排斥的病理生理学和使用病毒载体的基因递送的功效。

Research

JoVE Journal

Medicine

A Modified Method for Heterotopic Mouse Heart Transplantion

对于异位小鼠心脏移植技术的一种改进方法

Mice are often used as heart transplant donors and recipients in studies of transplant immunology due to the wide range of transgenic mice and reagents ...

科研

医学

Murine Heterotopic Heart Transplant Technique

小鼠异位心脏移植技术

It is now over forty years since this technique was first reported by Corry, Wynn and Russell. Although it took some years for other labs to become ...

Transplantation of Pulmonary Valve Using a Mouse Model of Heterotopic Heart Transplantation

肺动脉瓣使用异位心脏移植小鼠模型移植

Tissue engineered heart valves, especially decellularized valves, are starting to gain momentum in clinical use of reconstructive surgery with mixed ...

Rat Heterotopic Abdominal Heart/Single-lung Transplantation in a Volume-loaded Configuration

大鼠异位心脏腹部/在卷装配置单肺移植

Herein, we describe a novel technique for heterotopic abdominal heart-lung transplantation (HAHLT) in rats. The configuration of the transplant graft ...

Heterotopic Cervical Heart Transplantation in Mice

颈椎异位心脏移植小鼠

The heterotopic cervical heart transplantation in mice is a valuable tool in transplant and cardiovascular research. The cuff technique greatly simplifies ...

Heterotopic Renal Autotransplantation in a Porcine Model: A Step-by-Step Protocol

异位自体肾移植的猪模型：有步骤，分步协议

Kidney transplantation is the treatment of choice for patients suffering from end-stage renal disease. It offers better life expectancy and higher quality ...

A Pre-Clinical Porcine Model of Orthotopic Heart Transplantation

正交心脏移植的临床前猪模型

Fifty-years following the first successful report, cardiac transplantation remains the gold-standard treatment for eligible patients with advanced heart ...

A Simplified Model for Heterotopic Heart Valve Transplantation in Rodents

啮齿动物异位心脏瓣膜移植的简化模型

There is an urgent clinical need for heart valve replacements that can grow in children. Heart valve transplantation is proposed as a new type of ...

Left Lung Orthotopic Transplantation in a Juvenile Porcine Model for ESLP

ESLP幼猪模型中的左肺原位移植

Lung transplantation is the gold-standard treatment for end-stage lung disease, with over 4,600 lung transplantations performed worldwide annually. ...

A Modified Cuff Technique for Mouse Cervical Heterotopic Heart Transplantation Model

一种改进的袖带技术用于小鼠颈椎异位心脏移植模型

Cardiac allograft rejection limits the long-term survival of patients after heart transplantation. A mouse heart transplantation model is ideal for ...