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DOI :
10.3791/63118-v
January 19th, 2022
Chapters
0:05
Introduction
0:52
Cell Homogenization
3:56
Cell Membrane Solubilization
4:46
Cell Membrane Immobilization on IAM.PC.DD2 Particles
6:23
CMAC Column Packing
8:57
Results: Antibody Staining and Frontal Affinity Chromatography of Immobilized TrkB Receptors
11:02
Conclusion
该协议描述了用含有功能性跨膜滋养肌球蛋白激酶受体B蛋白的固定化细胞膜片段制备细胞膜亲和色谱(CMAC)柱。还解释了使用CMAC色谱柱鉴定与这些受体相互作用并存在于复杂天然混合物中的特化植物代谢物。
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