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DOI :
10.3791/63124-v
•
8:26 min
November 23rd, 2021
Chapters
0:05
Introduction
1:05
Measurement of Samples
1:38
Localization, Tracking, and Fluorescence Intensity Analysis of Single Molecules
4:03
Analysis and Filtering of Single-molecule Data
6:41
Plotting of Results and Further Analysis
7:17
Results: Spatiotemporal Analysis of Mobile, smFRET-based Probes Using Widefield Fluorescence Microscopy
7:45
Conclusion
本稿では、広視野蛍光顕微鏡を用いた移動式単分子フェルスター共鳴エネルギー伝達(smFRET)ベースのプローブの時空間解析方法を紹介する。新開発のソフトウェアツールキットは、正しいFRET効率や分子位置を含む移動プローブのsmFRET時間トレースを時間の関数として決定することを可能にします。
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