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DOI :
10.3791/63151-v
•
8:01 min
January 21st, 2022
Chapters
0:04
Introduction
0:35
Harvest of Rat Lungs
1:15
Cutting and Clipping the Lung Slices
2:25
Slice Decellularization
3:35
Slice Recellularization and Culture
6:20
Results: Visualization of the Structure of Decellularized Lung Scaffolds and Recellularized Engineered Lung Tissues (ELTs)
7:35
Conclusion
このプロトコールは、脱細胞化された精密切断肺スライスに肺胞上皮2型細胞、線維芽細胞、および内皮細胞を再移入することによって、再現性のある小規模の操作された肺組織を生成する方法を記載している。
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