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DOI :
10.3791/63263-v
March 30th, 2022
Chapters
0:04
Introduction
0:52
MMP Expression
3:46
MMP Purification
4:27
MMP Refolding
5:12
MMP Activation
5:46
Results: Purification and Activation of Hisx6-Pro-MMP-3cd From BL21(DE3) and R2DP Cells
6:38
Conclusion
그의 태그 정제, 투석 및 활성화는 박테리아에서 가용성, 활성 매트릭스 메탈로프로테이나제-3 촉매 도메인 단백질 발현의 수율을 증가시키기 위해 사용된다. 단백질 분획은 SDS-PAGE 겔을 통해 분석된다.
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