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DOI :
10.3791/63291-v
•
8:55 min
December 13th, 2021
Chapters
0:05
Introduction
0:40
Injection Needle Preparation
1:26
Injection Solution Preparation and Needle Loading
2:08
Embryo Preparation for Microinjection
4:34
Microinjecting Embryos
5:51
Imaging Embryos
6:38
Results: Representative Results for Organelle Dependent Trafficking in Drosophila Embryos
8:14
Conclusion
在早期的 果蝇 胚胎中,许多细胞器是运动的。原则上,它们 可以通过特定的荧光 探针进行现场成像,但蛋壳会阻止直接应用于胚胎。该协议描述了如何 通过 显微注射引入这种探针,然后 通过 粒子图像测速分析本体细胞器运动。
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