Name: pneumatically driven microfluidic platform for micro-particle concentration
Uploaded: 2022-02-01T14:15:00
Description: Watch this Scientific Journal Video about Pneumatically Driven Microfluidic Platform for Micro-Particle Concentration at JoVE.com

这项工作得到了韩国国家研究基金会（NRF）的支持，该基金由韩国政府（科学和信息通信技术部）资助。（编号NRF-2021R1A2C1011380）。

1. 颗粒浓缩微流控平台设计
<ol><li>设计气动微流体平台，该平台由一个用于 3D 流网络中流体流动的气动阀和三个用于筛网 （V）、流体 （Vf） 和颗粒 （Vp） 操作的气动阀组成（图 1）。 注意：Vs阻挡液体中的浓缩颗粒，Vf和Vp允许浓缩后释放流体和颗粒。三个气动端口提供来自流体/气动供应层（常开）的压缩空气和气动阀灯出口以驱动阀门。微流体通道网络...

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该平台提供了一种纯化和浓缩各种尺寸颗粒的简单方法。颗粒通过气动阀控制积聚和释放，并且没有观察到堵塞，因为没有被动结构。使用该装置，呈现三种尺寸的颗粒的浓度。然而，操作压力、浓缩所需的时间和速率可以根据器件尺寸、粒径放大倍率和Vs 18，20，21处的压力而变化。
当执行步骤3.1时，气?...

由于生物分析的重要性，微流体和生物医学微机电系统（BioMEMS）技术1，2 用于开发和研究用于纯化和收集微材料的装置2，3，4。粒子捕获分为主动或被动。主动式阱已用于作用于独立粒子的外部介电5、磁吞噬6、听觉7、视觉8或热9 力，从而能够精确控制其运动。然而，粒子和外力之间的相互作用是必需的;因此，吞吐量较低。在微流体系统中，控制流速非常重要，因为外力传递到目标颗粒。
一般来说，无源微流体器件在微通道10，11中具有微柱。颗粒通过与流动流体的相互作用进行过滤，这些设备易于设计且制造成本低廉。然而，它们在微柱中引起颗粒堵塞，因此已经开发出更复杂的装置来防止颗粒堵塞12。具有复杂结构的微流体装置通常适用于管理有限数量的颗粒13，14，15，16，17，18。
本文描述了一种制造和操作气动驱动的大颗粒浓度微流体平台的方法，该平台克服了上述缺点18。该平台可以通过变形和驱动积聚在弯曲微流体通道中的筛阀（Vs）板的薄隔膜层来阻挡和浓缩颗粒。颗粒积聚在弯曲的微流体通道中，并且浓缩的颗粒可以通过两个PDMS密封件的开/关阀18的驱动来排出工作流体来分离。这种方法使得可以处理有限数量的颗粒或浓缩大量小颗粒。诸如流速和压缩空气压力等工作条件可以防止不必要的电池损坏并提高电池捕获效率。

<table border="1" fo:keep-together.within-page="1" fo:keep-with-next.within-page="always">
	<tbody>
		<tr>
			<td>1.5 mm puncture</td>
			<td>Self procduction</td>
			<td>Self procduction</td>
			<td>This puncture was made by requesting a mold maker based on the Miltex&#174; Biopsy Punch with Plunger (15110-15) product.</td>
		</tr>
		<tr>
			<td>4 inch Silicon Wafer/SU-8 mold</td>
			<td>4science</td>
			<td>29-03573-01</td>
			<td>4 inch (100) Ptype silicon wafer/SU-8 mold</td>
		</tr>
		<tr>
			<td>Carboxyl Polystyrene Crosslinked Particle(24.9 &#956;m)</td>
			<td>Spherotech</td>
			<td>CPX-200-10</td>
			<td>Concentrated bead sample1</td>
		</tr>
		<tr>
			<td>Flow meter</td>
			<td>Sensirion</td>
			<td>SLI-1000</td>
			<td>Flow measurement</td>
		</tr>
		<tr>
			<td>High-speed camera</td>
			<td>Photron</td>
			<td>FASTCAM Mini</td>
			<td>Observation of concentration</td>
		</tr>
		<tr>
			<td>Hot plate</td>
			<td>As one</td>
			<td>HI-1000</td>
			<td>heating plate for curing of liquid PDMS</td>
		</tr>
		<tr>
			<td>KOVAX-SYRINGE 10 mL/Syringe</td>
			<td>Koreavaccine</td>
			<td>22G-10ML</td>
			<td>Fill the microfluidic channel with bubble-free demineralized water.</td>
		</tr>
		<tr>
			<td>Laboratory Conona treater/Atmospheric plasma</td>
			<td>Electro-Technic</td>
			<td>BD-20AC</td>
			<td>Chip bonding/atmospheric plasma</td>
		</tr>
		<tr>
			<td>Liquid polydimethylsiloxane, PDMS</td>
			<td>Dow Corning Inc.</td>
			<td>Sylgard 184</td>
			<td>Components of chip</td>
		</tr>
		<tr>
			<td>Microscope</td>
			<td>Olympus</td>
			<td>IX-81</td>
			<td>Observation of concentration</td>
		</tr>
		<tr>
			<td>PEEK Tubes</td>
			<td>SAINT-GOBAIN PPL CORP.</td>
			<td>AAD04103</td>
			<td>Inject or collect particles</td>
		</tr>
		<tr>
			<td>Polystyrene Particle(4.16 &#956;m)</td>
			<td>Spherotech</td>
			<td>PP-40-10</td>
			<td>Concentrated bead sample3</td>
		</tr>
		<tr>
			<td>Polystyrene Particle(8.49 &#956;m)</td>
			<td>Spherotech</td>
			<td>PP-100-10</td>
			<td>Concentrated bead sample2</td>
		</tr>
		<tr>
			<td>Pressure controller/&#956;flucon</td>
			<td>AMED</td>
			<td>&#956;flucon</td>
			<td>Control of air pressure</td>
		</tr>
		<tr>
			<td>Spin coater</td>
			<td>iNexus</td>
			<td>ACE-200</td>
			<td>spread the liquid PDMS on SU-8 mold</td>
		</tr>
	</tbody>
</table>

pneumatically driven microfluidic platform for micro-particle concentration

本文介绍了一种利用微流体平台制造和操作气动阀以控制颗粒浓度的方法。该平台具有具有弯曲流体通道和三个气动阀的三维（3D）网络，通过与聚二甲基硅氧烷（PDMS）的双相复制来创建网络，通道和空间。该装置根据气动阀控制的流体流速的瞬态响应按以下顺序运行：（1）样品上样，（2）样品阻塞，（3）样品浓度和（4）样品释放。颗粒被筛阀（Vs）板的薄隔膜层变形阻挡并积聚在弯曲的微流体通道中。工作流体通过两个开/关阀的驱动排出。由于该操作，所有各种放大倍率的粒子都被成功拦截并脱离。当应用该技术时，操作压力、浓缩所需的时间和浓缩速率可能会根据设备尺寸和粒度放大倍率而变化。

图8 显示了四级平台操作的流体速率的流速，如 表2所示。第一阶段是加载状态（状态）。该平台在所有阀门打开的情况下提供流体，并且工作流体（Qf）和颗粒（Qp）几乎相同，因为微流体通道网络表现出结构对称性。在第二阶段（b状态）中，压缩空气被输送到Vs以阻挡颗粒，并且随着Vs隔膜变形，流路变窄，并且通过液压阻力减小了在出口处测量的流速。Qf和Qp...

Watch this Scientific Journal Video about Pneumatically Driven Microfluidic Platform for Micro-Particle Concentration at JoVE.com

Pneumatically Driven Microfluidic Platform for Micro-Particle Concentration

本方案描述了一种气动微流体平台，可用于高效的微粒浓缩。

气动驱动的微流体平台，用于微颗粒浓缩

The present article introduces a method for fabricating and operating a pneumatic valve to control particle concentration using a microfluidic platform. ...

该协议描述了我们在气动驱动的微流体平台上制造和操作多颗粒浓度的方法，克服了颗粒堵塞和复杂结构等缺点。这种方法可以处理无限数量的颗粒，专注于大量的小颗粒，防止不必要的细胞损伤，并提高熵效率。由于生物分析的重要性，微流体和生物医学微电子机械系统技术用于开发和研究用于纯化和收集微材料的设备。首先，使用预先准备好的气动阀通道SU8模具复制PDMS层，以气动控制阀门。将10毫升液体PDMS和1毫升固化剂倒入准备好的气动阀通道模具中，并在90摄氏度下加热活化30分钟。PDMS结构固化后，分离SU8模具。冲孔三个1.5毫米气动口进入气动阀道，采用1.5毫米刺穿。将10毫升液体PDMS和1毫升固化剂倒入干净的SU8模具中。使用旋转涂层机以每分钟1500转的速度旋转涂层15秒，然后在90摄氏度下加热30分钟。在PDMS结构固化后分离SU8模具。用大气等离子体处理PDMS结构20秒。使用显微镜，根据通道结构对等离子体处理过的PDMS结构进行对准。通过将对齐的PDMS结构在90摄氏度下加热30分钟来粘合它们。采用1.5毫米的穿刺，在流体通道入口和出口内做一个直径为1.5毫米的孔，将气动通道内的部分粘合到薄膜片层上。使用两个SU8模具复制PDMS层的两侧，以形成微流体通道。正面使用弯曲的矩形微流体通道模具，背面使用微流体互连通道模具。将10毫升液体PDMS和1毫升固化剂倒入弯曲的矩形微流体通道模具中，并以每分钟1200转的速度旋转涂覆15秒，然后通过90摄氏度的热活化30分钟，为弯曲的流体室和流体通道创建模具。分离形成微流体通道的PDMS层。然后用大气等离子体处理20秒，通过粘接到玻璃晶圆上，制成覆盖密封通风壁的热活化模具。将三毫升液体PDMS倒入SU8模具的互连通道中。布置结构，用互连通道模具制作，在液体PDMS上微流体互连通道模具上。然后将叠加结构在130摄氏度下干燥30分钟。固化后，从微流体通道网络层中取出前SU8模具，并小心地剥离后部PDMS模具。将10毫升液体PDMS和1毫升固化剂倒入干净的SU8模具中，并在90摄氏度下加热活化30分钟。在PDMS结构固化后分离SU8模具。用大气等离子体处理PDMS微流体互连通道模具20秒。使用显微镜，根据通道结构对齐等离子体处理的PDMS结构。通过在90摄氏度下加热30分钟来粘合对齐的PDMS结构。根据通道结构对齐在此过程中制备的PDMS结构，并通过用大气等离子体处理20秒来粘合它们。使用10毫升注射器，用无气泡的脱矿质水填充微流体通道。要控制工作流体的压力和控制微珠流量的三个气动阀，请将具有四个或更多工作流体出口通道的精密压力控制器插入微流体平台。在蒸馏水中制备各种尺寸的羧基聚苯乙烯测试颗粒。为了控制工作流体的流速，用水填充玻璃瓶的一半，并将玻璃瓶盖连接到控制器输出通道和微阀。使用倒置显微镜，观察所有平台操作，并通过液体流量计测量出口处的操作流量。用颗粒阀在入口处的压力下注入颗粒或流体混合物。以15千帕斯卡的速度对CIV阀施加压力，以18千帕斯卡的速度对颗粒阀施加压力以启动阀门。当颗粒被浓缩时，仅对流体阀施加压力。流体的流速被划分为四级平台操作。第一阶段是加载状态。工作流体和颗粒几乎相同，因为微流体通道网络表现出结构对称性。第二阶段是阻塞状态。流路变窄，在出口处测量的流速因液压阻力而降低。第三阶段是集中状态。测得的QP接近于零，QF约为阻塞状态的1.42倍。最后阶段是发布状态。由此产生的流量和浓度率证明，由于流量变化，气动阀编程的顺序驱动工作良好。当CIV阀和颗粒阀关闭时，颗粒被浓缩并积聚在收集区域，并且当只有流体阀关闭时，所有收集的浓缩颗粒在四秒内被释放出来。该过程的一个重要部分是固化后部结构，其中PDMS层通过空气层的更多压力注入。变形的薄膜层突然被激活。该平台可用于非常浓缩，直的和悬浮的生物颗粒的自动预处理，因为操作不受物理颗粒特性的影响。

Research

JoVE Journal

Engineering

Fabrication, Operation and Flow Visualization in Surface-acoustic-wave-driven Acoustic-counterflow Microfluidics

制造，操作和表面声波驱动声逆流微流体流动可视化

Surface acoustic waves (SAWs) can be used to drive liquids in portable microfluidic chips via the acoustic counterflow phenomenon. In this video we ...

科研

工程学

Picoinjection of Microfluidic Drops Without Metal Electrodes

微流控的Picoinjection滴无金属电极

Existing methods for picoinjecting reagents into microfluidic drops require metal electrodes integrated into the microfluidic chip. The integration of ...

Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform

时间复用超解析技术成像的运动平台

We propose a method for increasing the resolution of an object and overcoming the diffraction limit of an optical system installed on top of a moving ...

Fabrication and Testing of Microfluidic Optomechanical Oscillators

制造和微流控光机振荡器测试

Cavity optomechanics experiments that parametrically couple the phonon modes and photon modes have been investigated in various optical systems including ...

Thermal Measurement Techniques in Analytical Microfluidic Devices

在分析微流体装置的热测量技术

Thermal measurement techniques have been used for many applications such as thermal characterization of materials and chemical reaction detection. ...

Capillary-based Centrifugal Microfluidic Device for Size-controllable Formation of Monodisperse Microdroplets

基于毛细管的离心微流体装置单分散微滴的大小可控的形成

Here, we demonstrate a simple method for the rapid production of size-controllable, monodisperse, W/O microdroplets using a capillary-based centrifugal ...

A High Performance Impedance-based Platform for Evaporation Rate Detection

蒸发速率检测一种基于阻抗高性能平台

This paper describes the method of a novel impedance-based platform for the detection of the evaporation rate. The model compound hyaluronic acid was ...

Solvent Bonding for Fabrication of PMMA and COP Microfluidic Devices

溶剂粘接的PMMA的制备和COP微流体装置

Thermoplastic microfluidic devices offer many advantages over those made from silicone elastomers, but bonding procedures must be developed for each ...

A Modular Microfluidic Technology for Systematic Studies of Colloidal Semiconductor Nanocrystals

胶体半导体纳米晶系统研究的模块化微流控技术

Colloidal semiconductor nanocrystals, known as quantum dots (QDs), are a rapidly growing class of materials in commercial electronics, such as light ...

Scalable Quantum Integrated Circuits on Superconducting Two-Dimensional Electron Gas Platform

超导二维电子气体平台上的可扩展量子集成电路

To form a coherent quantum transport in hybrid superconductor-semiconductor (S-Sm) junctions, the formation of a homogeneous and barrier-free interface ...