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DOI :
10.3791/63308-v
December 1st, 2021
Chapters
0:05
Introduction
1:12
Dye Loading Procedure
3:28
Video Capture with Confocal Microscopy
6:14
Video Processing
7:56
Video Analysis
9:55
Results: Mouse Nerve Terminals Loaded with the Ca2+ Indicator and Representative Trace of Calcium Signal
10:18
Conclusion
プロトコルは、切断された神経を通してマウス運動神経終末に蛍光カルシウム色素をロードする方法を説明しています。さらに、共焦点顕微鏡を使用して末梢神経終末の高速カルシウム過渡現象を記録するためのユニークな方法が提示されます。
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