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DOI :
10.3791/63320-v
•
10:31 min
February 3rd, 2022
Chapters
0:04
Introduction
1:39
Production of Recombinant Wild-Type Fluorescent Proteins and Selective Pressure Incorporation to Produce Fluorescent Proteins with Proline Analogs
4:08
Fluorescence Emission of Protein Variants
5:00
Denaturation and Refolding of EGFP Variants
6:38
Results: Effect of Atomic Substitutions in Variants of GFP
9:17
Conclusion
为了克服经典定点诱变的局限性,将具有特定修饰的脯氨酸类似物掺入几种荧光蛋白中。我们展示了氟替代氢或脯氨酸残基中的单双键(“分子手术”)如何影响基本的蛋白质特性,包括它们的折叠和与光的相互作用。
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