Research
Education
Sign In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Please note that all translations are automatically generated. Click here for the English version.
DOI :
10.3791/63333-v
February 18th, 2022
Chapters
0:04
Introduction
0:55
Preparation of Buffer, Tissue Sample, and Solution
2:15
Total Protein Extraction
3:42
SDS-PAGE and Western Blot Analysis
4:39
Ammonium Sulfate Precipitation
6:28
Ionic Exchange Chromatography with FPLC
8:14
Protein Extraction Using Defined Sub-Protocols for Ammonium Sulfate-Precipitation and FPLC
9:32
Results: Extraction and Purification of FAHD1 Protein from Swine Kidney and Mouse Liver
11:24
Conclusion
このプロトコールは、ブタ腎臓およびマウス肝臓からフマリアセトアセテート加水分解酵素ドメイン含有タンパク質1(FAHD1)を抽出する方法を記載する。列挙された方法は、目的の他のタンパク質に適合させ、他の組織に対して改変してもよい。
Tags
-- Views
Related Videos
Privacy
Terms of Use
Policies
Contact Us
Recommend to library
JoVE NEWSLETTERS
JoVE Journal
Methods Collections
JoVE Encyclopedia of Experiments
Archive
JoVE Core
JoVE Business
JoVE Science Education
JoVE Lab Manual
Faculty Resource Center
Authors
Overview
Publishing Process
Editorial Board
Scope and Policies
Peer Review
FAQ
Submit
Librarians
Testimonials
Subscriptions
Access
Resources
Library Advisory Board
ABOUT JoVE
Leadership
Copyright © 2024 MyJoVE Corporation. All rights reserved