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DOI :
10.3791/63365-v
•
6:49 min
December 17th, 2021
Chapters
0:04
Introduction
0:42
Growth of an Age-Synchronized Population of C. elegans
1:12
Sample Preparation of C. elegans in a Multiwell Plate
1:43
Image Acquisition on the High-Throughput Confocal Microscope
2:45
Automated Inclusion Counting Using CellProfiler
4:07
Global Fitting of Inclusion Count Data Using AmyloFit
5:39
Results: Fitting the Data to a Mathematical Model in AmyloFit
6:16
Conclusion
여기에서, 선충류 예쁜꼬마선충에서의 단백질 응집 동역학의 분석을 위한 방법이 제시 된다. 연령 동기화 된 집단의 동물은 서로 다른 시점에서 이미지화되고 CellProfiler에서 반자동 포함 계수가 수행되고 AmyloFit의 수학적 모델에 적합합니다.
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