Name: murine skin transplantation
Uploaded: 2008-01-16T10:34:00
Description: Watch this Scientific Journal Video about Murine Skin Transplantation at JoVE.com

<p>美国国立卫生研究院免疫学练金的T32人工智能60573（KRG）和美国国立卫生研究院授予通用汽车41514（MDC） </p>

<p class="jove_step">实验开始之前： </p><ul><li>获取IACUC批准</li><li>消毒手术器械和Q -秘诀</li></ul><p class="jove_step">一，采收供体组织</p><ol><li>安乐死捐助鼠标（按照机构的SOP） </li><li>湿漉漉的头发从耳根用酒精回</li><li>收获基地耳捐助皮肤</li><li>把组织上的冰板</li><li>轻轻拉开分割的耳朵：捏腹侧和背侧组织双方在基地与鼠齿钳耳;组织;浮动背侧组织，内部表面向下，在PBS冰;丢弃胶原腹皮瓣</li></ol><p class="jove_step">二。?...

<p>在这个视频文章表明，皮肤移植模型是一个监测异体T细胞反应的快速和简便的方法。异体皮移植，具有广泛的应用，并已被用来检测各种免疫抑制剂和耐受的协议，以及调查各种阻断抗体的生物效应的效率。 </p>

<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd">
<html xmlns="http://www.w3.org/1999/xhtml" xml:lang="en" lang="en">	
		
		<div>
		<table border="1">
		<thead>
		<tr>
		<th>Material Name</th>
		<th>Type</th>
		<th>Company</th>
		<th>Catalogue Number</th>
		<th>Comment</th>
		</tr>
		</thead>
		<tbody>
		
<tr>
<td>Micro-dissecting forceps (curved)</td>
<td>&nbsp;</td>
<td>Sigma</td>
<td>F4142</td>
<td>&nbsp;</td>
<tr>
<td>Rat-tooth forceps (1x2 teeth, straight)</td>
<td>&nbsp;</td>
<td>Fine Science Tools</td>
<td>11053-10</td>
<td>n=2</td>
</tr>
<tr>
<td>Strabismus scissors (blunt end)</td>
<td>&nbsp;</td>
<td>Fine Science Tools</td>
<td>14074-11</td>
<td>&nbsp;</td>
<tr>
<td>Iris forceps (curved)</td>
<td>&nbsp;</td>
<td>Fine Science Tools</td>
<td>11065-07</td>
<td>&nbsp;</td>
<tr>
<td>Spring scissors (angled)</td>
<td>&nbsp;</td>
<td>Fine Science Tools</td>
<td>15006-09</td>
<td>&nbsp;</td>
<tr>
<td>Halsey micro needle holder</td>
<td>&nbsp;</td>
<td>Fine Science Tools</td>
<td>12500-12</td>
<td>&nbsp;</td>
<tr>
<td>10 cm plates</td>
<td>&nbsp;</td>
<td>Corning</td>
<td>430167</td>
<td>&nbsp;</td>
<tr>
<td>PBS</td>
<td>&nbsp;</td>
<td>Cellgro</td>
<td>21-040-CV</td>
<td>&nbsp;</td>
<tr>
<td>Bupernex (Buprenorphine hydrochloride)</td>
<td>&nbsp;</td>
<td>Reckitt Bencikiser Healthcare</td>
<td>517201</td>
<td>&nbsp;</td>
<tr>
<td>Insulin syringes (29 Ga)</td>
<td>&nbsp;</td>
<td>Monoject</td>
<td>8881 600145</td>
<td>&nbsp;</td>
<tr>
<td>Q-tips</td>
<td>&nbsp;</td>
<td>Solon</td>
<td>362</td>
<td>&nbsp;</td>
<tr>
<td>Single edge razor blades (#9)</td>
<td>&nbsp;</td>
<td>Smith</td>
<td>67-0238</td>
<td>&nbsp;</td>
<tr>
<td>Silk suture (2-0; PS-2)</td>
<td>&nbsp;</td>
<td>Ethicon</td>
<td>583H</td>
<td>&nbsp;</td>		</tbody>
		</table>
		</div>
	

murine skin transplantation

<p class="jove_content">皮肤移植的最严格和最不发达国家在技术上具有挑战性的车型之一，是一个标准的方法来检测主机的T细胞应答的MHC不同的捐助抗原。此视频文章的目的是向观众提供了一步一步的视觉使用皮肤移植小鼠模型示范。该协议分为5个主要部分组成：1）皮肤收获捐助; 2）准备移植; 3）皮肤移植; 4）绷带拆除和移植排斥反应的监测; 5）有用的提示。一旦精通，程序本身应采取&lt;10分钟来执行。</p>

Watch this Scientific Journal Video about Murine Skin Transplantation at JoVE.com

Murine Skin Transplantation

<p class="jove_content">异体皮移植是一个标准的模型来检测主机的T细胞应答的MHC不同的捐助抗原。此视频文章执行BALB/c--&gt; C57BL / 6皮肤移植的每个步骤提供了一个可视化教程。</p>

小鼠皮肤移植

As one of the most stringent and least technically challenging models, skin transplantation is a standard method to assay host T cell responses to ...

Research

JoVE Journal

Biology

Heterotopic Heart Transplantation in Mice

小鼠异位心脏移植

The mouse heterotopic heart transplantation has been used widely since it was introduced by Drs. Corry and Russell in 1973. It is particularly valuable ...

科研

生物学

Small Bowel Transplantation In Mice

小鼠小肠移植

Since 1990, the development of tacrolimus-based immunosuppression and improved surgical techniques, the increased array of potent immunosuppressive ...

Murine Renal Transplantation Procedure

小鼠肾移植程序

Renal orthotopic transplantation in mice is a technically challenging procedure. Although the first kidney transplants in mice were performed by Russell ...

Generation of Human CD40-activated B cells

人CD40激活的B细胞的生成

CD40-activated B cells (CD40-B cells) have been identified as an alternative source of immuno-stimulatory antigen-presenting cells (APC) for cancer ...

Generating Chimeric Zebrafish Embryos by Transplantation

移植嵌合斑马鱼胚胎生成

One of the most powerful tools used to gain insight into complex developmental processes is the analysis of chimeric embryos. A chimera is defined as an ...

Planarian Immobilization, Partial Irradiation, and Tissue Transplantation

涡固定，局部照射，组织移植

The planarian, a freshwater flatworm, has proven to be a powerful system for dissecting metazoan regeneration and stem cell biology1,2. Planarian ...

Studying Proteolysis of Cyclin B at the Single Cell Level in Whole Cell Populations

在整个细胞群在单细胞水平上研究细胞周期蛋白B的蛋白水解

Equal distribution of chromosomes between the two daughter cells during cell division is a prerequisite for guaranteeing genetic stability 1. Inaccuracies ...

Isolation, Culture, and Transplantation of Muscle Satellite Cells

分离，培养和移植肌卫星细胞

Muscle satellite cells are a stem cell population required for postnatal skeletal muscle development and regeneration, accounting for 2-5% of sublaminal ...

An Immunofluorescent Method for Characterization of Barrett&#8217;s Esophagus Cells

An Immunofluorescent Method for Characterization of BarrettÃƒÂ¢Ã¢â€šÂ¬Ã¢â€žÂ¢s Esophagus Cells

免疫荧光法Barrett食管细胞的表征

Esophageal adenocarcinoma (EAC) has an overall survival rate of less than 17% and incidence of EAC has risen dramatically over the past two decades. One ...

Protocol for Isolation of Primary Human Hepatocytes and Corresponding Major Populations of Non-parenchymal Liver Cells

协议主要人肝细胞的分离和相应的非实质肝细胞的主要种群

Beside parenchymal hepatocytes, the liver consists of non-parenchymal cells (NPC) namely Kupffer cells (KC), liver endothelial cells (LEC) and hepatic ...