Name: murine skin transplantation
Uploaded: 2008-01-16T10:34:00
Description: Watch this Scientific Journal Video about Murine Skin Transplantation at JoVE.com

<p> NIH immunologi utbildningsstipendium T32 AI 60.573 (KRG) och NIH ge GM 41.514 (MDC) </p>

<p class="jove_step"> Före inledandet av experiment: </p><ul><li> Skaffa IACUC godkännande </li><li> Sterilisera kirurgiska instrument och Q-tips </li></ul><p class="jove_step"> I. Harvest Donor Tissue </p><ol><li> Euthanize givare mus (i enlighet med institutionens SOP) </li><li> Vått hår tillbaka från basen på örat med alkohol </li><li> Harvest givare huden genom att skära örat av vid basen </li><li> Placera vävnad i platta på is </li><li> Split öra: nypa ventrala och dorsala sidor av vävnaden vid basen av örat med rått-tanden ...

<p> Såsom visats i denna video-artikeln är hudtransplantation modell en snabb och enkel metod för att övervaka allogen T-cell svar. Allogen hud transplantation har ett brett användningsområde och har utnyttjats till analys effektiviteten i en mängd olika immunsuppressiva medel och tolerans protokoll induktion, samt att undersöka de biologiska effekterna av olika blockering antikroppar. </p>

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murine skin transplantation

<p class="jove_content">Som en av de hårdaste och minst tekniskt utmanande modeller är hud transplantation en standardmetod till T-analysen värdcellen svar på MHC-olika givare antigener. Syftet med denna video-artikel är att ge betraktaren med en steg-för-steg visuell demonstration av hud transplantation med musen modellen. Protokollet är uppdelat i fem delar: 1) skörd givare huden, 2) förbereda mottagaren för transplantation, 3) hudtransplantation, 4) bandage borttagning och övervakning avstötning, 5) tips. När skickliga, bör förfarandet själv ta &lt;10 min att utföra.</p>

Watch this Scientific Journal Video about Murine Skin Transplantation at JoVE.com

Murine Skin Transplantation

<p class="jove_content">Allogen hud transplantation är en standardmodell till T-analysen värdcellen svar på MHC-disparata givare antigener. Denna video-artikeln ger en visuell handledning för varje steg involverade i att utföra en BALB/c--&gt; C57BL / 6 hudtransplantation.</p>

Murina hudtransplantation

As one of the most stringent and least technically challenging models, skin transplantation is a standard method to assay host T cell responses to ...

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