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DOI :
10.3791/63413-v
May 24th, 2022
Chapters
0:04
introduction
0:55
Generation and Surgical Implantation of the Mammary Imaging Window (MIW)
4:13
Positioning and Maintaining Mouse on the Microscope Stage for Imaging
5:52
Set Up for 4D, Intensity-Based, Label-Free Intravital Imaging of Dynamic Cell Behavior
8:02
Fluorescence Lifetime Imaging (FLIM) of NAD(P)H
8:43
Results: Intravital Imaging of the Mammary Tumor Microenvironment
10:15
Conclusion
ここで説明するイントラバイタルイメージング法は、コラーゲンの第二高調波発生と代謝補因子NAD(P)Hからの内因性蛍光を利用して、標識されていない腫瘍微小環境を腫瘍、間質、血管区画に非侵襲的にセグメント化し、4Dインタビタル内画像の詳細な解析を行います。
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