Name: application of optical coherence tomography to a mouse model of retinopathy
Uploaded: 2022-01-12T14:30:00
Description: Watch this Scientific Journal Video about Application of Optical Coherence Tomography to a Mouse Model of Retinopathy at JoVE.com

Project Source: Natural Science Foundation of Guangdong Province (2018A0303130306). The authors would like to thank the Ophthalmic Research Laboratory, Joint Shantou International Eye Center of Shantou University and the Chinese University of Hong Kong for funding and materials.

The operations were performed following the Statement on the Use of Animals in Ophthalmic and Vision research from the Association for Research in Vision and Ophthalmology. The experimental design was approved by the institutional animal Ethics Committee (Medical Ethics Committee of JSIEC, EC 20171213(4)-P01). Two-month-old C57BL/6J mice and Vldlr knockout mice were used in this study. There were 7 mice in each group, all of which were female and weighed 20 g to 24 g.
1. Experimental co...

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In this study, OCT imaging using a small-animal retinal imaging system was applied to evaluate retinal changes in Vldlr knockout mice, which demonstrate incomplete posterior vitreous detachment, subretinal neovascularization, and retinal thickness thinning. OCT is a noninvasive imaging method to examine the condition of the retina in vivo. Most OCT devices are designed for human eye examination. The size of the hardware equipment, the setting of the focal length, the setting of the system parameters, an...

Optical coherence tomography (OCT) is an imaging technique that can provide in vivo high resolution and crosssectional imaging for tissue1,2,3,4,5,6,7,8, especially for the noninvasive examination in the retina9,10,11,12. It can also be used to quantify some important biomarkers, such as retinal thickness and retinal nerve fiber layer thickness. The principle of OCT is optical coherence reflectometry, which obtains crosssectional tissue information from the coherence of light reflected from a sample and converts it into a graphic or digital form through a computer system7. OCT is widely used in ophthalmology clinics as an essential tool for diagnosis, follow-up, and management for patients with retinal disorders. It can also provide insight into the pathogenesis of retinal diseases.
In addition to clinical applications, OCT has also been used in animal studies. Although pathology is the gold standard of morphological characterization, OCT has the advantage of noninvasive in vivo imaging and longitudinal follow-up. Furthermore, it has been shown that OCT is well correlated with histopathology in retinopathy animal models11,13,14,15,16,17,18,19,20. The mouse is the most commonly used animal in biomedical studies. However, its small eyeballs pose a technical challenge to conducting OCT imaging in mice.
Compared to the OCT first used for retinal imaging in mice21,22, OCT in small animals has now been optimized with respect to hardware and software systems. For example, OCT, in combination with the tracker, significantly reduces the signal-to-noise ratio; OCT software system upgrades allow more retinal layers to be detected automatically; and the integrated DLP beamer helps to reduce the motion artifacts.
Very-low-density lipoprotein receptor (Vldlr) is a transmembrane protein in endothelial cells. It is expressed on retinal vascular endothelial cells, retinal pigment epithelial cells, and around the outer limiting membrane23,24. Subretinal neovascularization is the phenotype of Vldlr knockout mice23. Therefore, Vldlr knockout mice are used to investigate the pathogenesis and potential therapy of subretinal neovascularization. This article demonstrates the application of OCT imaging to detect retinal lesions in Vldlr knockout mice, hoping to provide some technical reference for retinopathy research in small animal models.

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			<td>Volk Optical,Inc, Mentor, OH</td>
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			<td>Accessory belonging to the RETImap</td>
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			<td>Double aspheric 60-Dpt glass lens</td>
			<td>Volk Optical,Inc, Mentor, OH</td>
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			<td>Accessory belonging to the RETImap</td>
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			<td>Electric heating blanket</td>
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			<td>CW-DRT-01</td>
			<td>50 x 35 cm</td>
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			<td>Injection syringe (1 mL)</td>
			<td>Kaile</td>
			<td></td>
			<td>0.45 x 16RWLB</td>
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			<td>Levofloxacin Hydrochloride Eye Gel</td>
			<td>EBE PHARMACEUTICAL Co.LTD</td>
			<td></td>
			<td>5 g: 0.015 g</td>
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			<td>Medical sodium hyaluronate gel</td>
			<td>Alcon</td>
			<td>16H01E</td>
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			<td>Microliter syringes</td>
			<td>Shanghai high pigeon industry and trade co., LTD</td>
			<td>Q31/0113000236C001-2017</td>
			<td>50 &#181;L</td>
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			<td>Povidone iodine solution</td>
			<td>Guangdong medihealth pharmaceutical Co.,LTD</td>
			<td></td>
			<td>100 mL</td>
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			<td>RETImap</td>
			<td>ROLAND CONSULT</td>
			<td>19-99_50-2.1_1.2E</td>
			<td>cSLO/ERG/VEP/FA/OCT/GFP</td>
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			<td>OSMO POCKET OT110</td>
			<td>INS1005-1S</td>
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			<td>Santen Pharmaceutical Co.,LTD</td>
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			<td>5 mg/mL</td>
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			<td>Xylazin</td>
			<td>Sigma</td>
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			<td>5 g</td>
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			<td>Zoletil 50</td>
			<td>Virbac.S.A</td>
			<td>7FRPA</td>
			<td>Tiletamine 125 mg + Zolazepam 125 mg</td>
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application of optical coherence tomography to a mouse model of retinopathy

Optical coherence tomography (OCT) offers a noninvasive method for the diagnosis of retinopathy. The OCT machine can capture retinal crosssectional images from which the retinal thickness can be calculated. Although OCT is widely used in clinical practice, its application in basic research is not as prevalent, especially in small animals such as mice. Because of the small size of their eyeballs, it is challenging to conduct fundus imaging examinations in mice. Therefore, a specialized retinal imaging system is required to accommodate OCT imaging on small animals. This article demonstrates a small-animal-specific system for OCT examination procedures and a detailed method for image analysis. The results of retinal OCT examination of very-low-density lipoprotein receptor (Vldlr) knockout mice and C57BL/6J mice are presented. The OCT images of C57BL/6J mice showed retinal layers, while those of Vldlr knockout mice showed subretinal neovascularization and retinal thinning. In summary, OCT examination could facilitate the noninvasive detection and measurement of retinopathy in mouse models.

Thanks to the high-resolution scans of OCT, the layers of the mouse retina can be observed, and abnormal reflections and their exact locations can be identified. The retinal OCT images of Vldlr knockout mice and C57BL/6J mice were compared in this study. The OCT images of all C57BL/6J mice showed various retinal layers with different reflectivity, and the demarcation was clear (Figure 8D). In contrast, all Vldlr knockout mice showed abnormal, hyperreflective lesions on the ...

Watch this Scientific Journal Video about Application of Optical Coherence Tomography to a Mouse Model of Retinopathy at JoVE.com

Application of Optical Coherence Tomography to a Mouse Model of Retinopathy

Here, we describe an in vivo imaging technique using optical coherence tomography to facilitate the diagnosis and quantitative measurement of retinopathy in mice.

Optical coherence tomography (OCT) offers a noninvasive method for the diagnosis of retinopathy. The OCT machine can capture retinal crosssectional images ...

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