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Inoculation Strategies to Infect Plant Roots with Soil-Borne Microorganisms

5.4K Views

08:16 min

March 1st, 2022

March 1st, 2022

5,400 Views

0:04

Introduction

0:54

An in Vitro Inoculation System Based on Petri Dishes

2:55

An in Vitro Inoculation System Based on Plastic Cups

4:23

A Soil Based Inoculation System in Pots

6:07

Results: Infection of Arabidopsis Roots

7:29

Conclusion

Transcript

Although countless microbes colonize plant roots, these interactions are difficult to analyze as they happen underground. To facilitate this, this protocol collection provides an overview of strategies to inoculate plant roots with soil-borne micr

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This protocol presents a detailed summary of strategies to inoculate plant roots with soil-borne microbes. Exemplified for the fungi Verticillium longisporum and Verticillium dahliae, three different root infection systems are described. Potential applications and possible downstream analyses are highlighted, and advantages or disadvantages are discussed for each system.

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