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A Method to Preserve Wetland Roots and Rhizospheres for Elemental Imaging

DOI :

10.3791/62227-v

February 15th, 2021

February 15th, 2021

2,980 Views

1Department of Plant and Soil Sciences, University of Delaware, 2National Synchrotron Radiation Lightsource-II, Brookhaven National Laboratory

We describe a protocol to sample, preserve, and section intact roots and the surrounding rhizosphere soil from wetland environments using rice (Oryza sativa L.) as a model species. Once preserved, the sample can be analyzed using elemental imaging techniques, such as synchrotron X-ray fluorescence (XRF) chemical speciation imaging.

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