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DOI :
10.3791/63481-v
•
8:44 min
February 3rd, 2022
Chapters
0:05
Introduction
0:38
Concentrating the Vesicle Sample
2:58
Vesicle Isolation and Purification
4:47
Characterization of the Isolated Vesicles Using Transmission Electron Microscopy
5:22
Characterization of the Isolated Vesicles Using Nanoparticle Tracking Analysis
6:38
Results: An Overview of the Cyanobacterial Vesicle Isolation
7:42
Conclusion
본 프로토콜은 시아노박테리아 배양물로부터의 세포밖 소포의 분리, 농도 및 특성화에 대한 상세한 설명을 제공한다. 서로 다른 규모의 문화에서 소포를 정화하기위한 접근법, 방법론 간의 절충점 및 현장 샘플 작업을위한 고려 사항도 논의됩니다.
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