Name: a minimally invasive, fast spinal cord lateral hemisection technique for modeling open spinal cord injuries in rats
Uploaded: 2022-03-23T13:00:00
Description: Watch this Scientific Journal Video about A Minimally Invasive, Fast Spinal Cord Lateral Hemisection Technique for Modeling Open Spinal Cord Injuries in Rats at JoVE.com

The authors wish to thank Gergely &#193;ngy&#225;n for the original artwork. This research work was funded by Semmelweis University, Budapest, Hungary. This study was also supported by the Hungarian Human Resources Development Operational Program (EFOP-3.6.2-16-2017-00006). Additional support was received from the Thematic Excellence Programme (2020-4.1.1.-TKP2020) of the Ministry for Innovation and Technology in Hungary, within the framework of the Therapy thematic program of Semmelweis University.

All animal procedures were carried out according to the EU Directive (2010/63/EU) and were approved by the animal ethics committee of the Hungarian National Food Chain Safety Office (PEI/001/2894-11/2014). All applicable institutional and governmental regulations concerning the ethical use of animals were followed during this study.
1. Preparation before surgery
<ol>
	<li>Sterilize all instruments used during the procedure (see the Table of Materials) and di...

<ol>
	<li>Failli, V., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Functional+neurological+recovery+after+spinal+cord+injury+is+impaired+in+patients+with+infections.">Functional neurological recovery after spinal cord injury is impaired in patients with infections.</a> Brain. 135, 3238-3250 (2012).</li><li>Guan, B., Chen, R., Zhong, M., Liu, N., Chen, Q. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Protective+effect+of+Oxymatrine+against+acute+spinal+cord+injury+in+rats+via+modulating+oxidative+stress,+inflammation+and+apoptosis.">Protective effect of Oxymatrine against acute spinal cord injury in rats via modulating oxidative stress, inflammation and apoptosis.</a> Metabolic Brain Disease. 35 (1), 149-157 (2020).</li><li>Kjell, J., Olson, L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Rat+models+of+spinal+cord+injury:+from+pathology+to+potential+therapies.">Rat models of spinal cord injury: from pathology to potential therapies.</a> Disease Models &amp; Mechanisms. 9 (10), 1125-1137 (2016).</li><li>Minakov, A. N., Chernov, A. S., Asutin, D. S., Konovalov, N. A., Telegin, G. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Experimental+models+of+spinal+cord+injury+in+laboratory+rats.">Experimental models of spinal cord injury in laboratory rats.</a> Acta Naturae. 10 (3), 4-10 (2018).</li><li>Borb&#233;ly, Z., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effect+of+rat+spinal+cord+injury+(hemisection)+on+the+ex+vivo+uptake+and+release+of+[3H]noradrenaline+from+a+slice+preparation.">Effect of rat spinal cord injury (hemisection) on the ex vivo uptake and release of [3H]noradrenaline from a slice preparation.</a> Brain Research Bulletin. 131, 150-155 (2017).</li><li>Brown, A. R., Martinez, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Thoracic+spinal+cord+hemisection+surgery+and+open-field+locomotor+assessment+in+the+rat.">Thoracic spinal cord hemisection surgery and open-field locomotor assessment in the rat.</a> Journal of Visualized Experiments: JoVE. (148), e59738 (2019).</li><li>Taoka, Y., Okajima, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Spinal+cord+injury+in+the+rat.">Spinal cord injury in the rat.</a> Progress in Neurobiology. 56 (3), 341-358 (1998).</li><li>Hou, S., Saltos, T. M., Iredia, I. W., Tom, V. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Surgical+techniques+influence+local+environment+of+injured+spinal+cord+and+cause+various+grafted+cell+survival+and+integration.">Surgical techniques influence local environment of injured spinal cord and cause various grafted cell survival and integration.</a> Journal of Neuroscience Methods. 293, 144-150 (2018).</li><li>Mattucci, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Development+of+a+traumatic+cervical+dislocation+spinal+cord+injury+model+with+residual+compression+in+the+rat.">Development of a traumatic cervical dislocation spinal cord injury model with residual compression in the rat.</a> Journal of Neuroscience Methods. 322, 58-70 (2019).</li><li>Ahmed, R. U., Alam, M., Zheng, Y. P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Experimental+spinal+cord+injury+and+behavioral+tests+in+laboratory+rats.">Experimental spinal cord injury and behavioral tests in laboratory rats.</a> Heliyon. 5 (3), 01324 (2019).</li><li>Ashammakhi, N., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Regenerative+therapies+for+spinal+cord+injury.">Regenerative therapies for spinal cord injury.</a> Tissue Engineering. Part B, Reviews. 25 (6), 471-491 (2019).</li></ol>

This minimally invasive spinal cord injury technique was developed when studying spinal cord-injured rats, and the team was faced with problems arising from the surgery itself (bone splinters from the laminectomy causing compression and damaging the spinal cord, surgery taking too long, slow healing of a large bone wound). By eliminating the laminectomy, the procedure became much faster (11 min vs. 35 min), the structure of the vertebral canal remained intact, the bone wound was much smaller, and there were no bone splin...

Spinal cord injuries (SCIs) are unfortunately prevalent injuries in humans. SCIs can be complicated in different ways, for example, by infections, and it is clinically important to study these injuries1. Because there is no single, definite cure for SCIs, animal models are still needed to further the understanding of researchers and advance possible treatments2,3. Although closed injuries are most commonly modeled (compression and contusion), it is clinically important to understand lacerations, which can only be modeled in open injuries4. Open-wound models using transection or hemisection can be used to demonstrate a more precise localization of a wound compared to closed injury models, owing to the nature of the injury (contusion vs. surgical cut). Open-wound experiments can shed light on more specific neuronal injuries in a controlled, reliable, and replicable way5. The complete or partial transection of the spinal cord is a widely used open-wound technique and can be viewed in detail in the article by Brown and Martinez6.
When studying open spinal cord injury in rats, several animals presented problems that arose from the surgery: bone splinters from the laminectomy became embedded in the spinal cord and caused swelling; the larger bone wound needed a long time to heal; the surgery took too long. An alternate surgical technique was developed to eliminate these problems. The goal was to develop a faster technique that is gentler for the animal. This newly developed technique is much faster than traditional SCI techniques. The surgical approach is minimally invasive, resulting in a smaller bone wound while eliminating problems arising from the laminectomy.
All open-wound techniques involve opening the dura7. Several recent studies have examined different, newly developed techniques, aiming to improve the previous methods8,9. Even though the opening of the dura cannot be excluded using this new technique, it causes a smaller wound on the dura while offering a reliable, controlled injury of the spinal cord. Consulting the literature on spinal cord injury techniques, many authors tried to minimize the time of surgery by implementing minor changes to the original technique10. Laminectomy is always part of these surgical procedures, although it is time-consuming and requires a larger bone wound to be made6. This surgical technique can be appropriate for researchers using open wound spinal cord injury models, specifically complete transection or lateral hemisection performed in the intervertebral spaces (Figure 1).

<table border="1" fo:keep-together.within-page="1" fo:keep-with-next.within-page="always">
	<tbody>
		<tr>
			<td>Augmentin (1,000 mg/200 mg powder)</td>
			<td>GlaxoSmithKline, UK</td>
			<td></td>
			<td>One-time dose of s.c. antibiotics prophylactically (10 mg of amoxicillin and 2 mg clavulanic acid; Augmentin 1,000 mg/200 mg powder). Every day following surgery, 10 mg of amoxicillin and 2 mg of clavulanic acid (Augmentin 1,000 mg/200 mg powder) per day per animal</td>
		</tr>
		<tr>
			<td>Betadine</td>
			<td>EGIS, Hungary</td>
			<td></td>
			<td>Disinfect the skin of the surgical area using a povidone-iodine solution</td>
		</tr>
		<tr>
			<td>Calypsol (50 mg/mL)</td>
			<td>Richter Gedeon, Hungary</td>
			<td></td>
			<td>Anesthesia: combination of ketamine 80 mg/kg and xylazine 8 mg/kg intramuscularly</td>
		</tr>
		<tr>
			<td>CP XYLAZIN 2% (20 mg/mL)</td>
			<td>Produlab Pharma B.V., the Netherlands</td>
			<td></td>
			<td>Anesthesia: combination of ketamine 80 mg/kg and xylazine 8 mg/kg intramuscularly</td>
		</tr>
		<tr>
			<td>Dental bone forceps</td>
			<td>Dentech, Hungary</td>
			<td>BS 0127</td>
			<td>Remove the spinous processes of the 13th thoracic vertebra and the 1st lumbar vertebra using dental bone forceps</td>
		</tr>
		<tr>
			<td>dental surgical micromotor</td>
			<td>W&#38;H, Austria</td>
			<td>MF-TECTORQUE</td>
			<td>Using a dental surgical micromotor, a laminectomy is performed at the L1 vertebra</td>
		</tr>
		<tr>
			<td>optical microscope</td>
			<td>Zeiss, Germany</td>
			<td>OPMI19-FC</td>
			<td>Control the procedure by viewing an enlarged (16x magnification) microscopic image</td>
		</tr>
		<tr>
			<td>physiological saline solution (0.9% NaCl)</td>
			<td>Fresenius Kabi, Germany</td>
			<td></td>
			<td>Keep the rat&#39;s eyes moist throughout the entire anesthesia using physiological saline solution drops (reapply as necessary)</td>
		</tr>
		<tr>
			<td>raspatorium</td>
			<td>Dentech, Hungary</td>
			<td>FK 1164</td>
			<td>Dissect the muscles attached to the vertebrae with the aid of a raspatorium, until all the spinal ligaments are visible.</td>
		</tr>
		<tr>
			<td>retractor</td>
			<td>Dentech, Hungary</td>
			<td>RT 1253</td>
			<td></td>
		</tr>
		<tr>
			<td>scalpel</td>
			<td>Dentech, Hungary</td>
			<td>BB 173</td>
			<td></td>
		</tr>
		<tr>
			<td>scalpel</td>
			<td>Dentech, Hungary</td>
			<td>BB 184</td>
			<td></td>
		</tr>
		<tr>
			<td>scalpel blade 12</td>
			<td>B. Braun, Germany</td>
			<td>12</td>
			<td></td>
		</tr>
		<tr>
			<td>scalpel blade 20</td>
			<td>B. Braun, Germany</td>
			<td>20</td>
			<td></td>
		</tr>
		<tr>
			<td>sterile cut gauze 10 x 10 cm</td>
			<td>Sterilux, Hartmann, Germany</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>sutures (monofilament, synthetic; absorbable and nonabsorbable), size: 4-0</td>
			<td>B. Braun, Germany</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>tweezer (13 cm)</td>
			<td>Dentech, Hungary</td>
			<td>BD 1555</td>
			<td></td>
		</tr>
		<tr>
			<td>tweezer (delicate tissue forceps)</td>
			<td>Dentech, Hungary</td>
			<td>BD 1670</td>
			<td></td>
		</tr>
	</tbody>
</table>

a minimally invasive, fast spinal cord lateral hemisection technique for modeling open spinal cord injuries in rats

Open spinal cord injury techniques modeling laceration-like injuries are time-consuming and invasive because they involve laminectomy. This new technique eliminates laminectomy by removing two spinous processes and lifting, then tilting the caudal vertebral arch. The surgical area opens up without the need for laminectomy. Lateral hemisection is then performed with direct visible control under a microscope. The trauma is minimized, requiring only a small bone wound. 
This technique has several advantages: it is faster and, therefore, less of a burden for the animal, and the bone wound is smaller. Because the laminectomy is eliminated, there is less chance for unwanted injury to the spinal cord, and there are no bone splinters that can cause problems (bone splinters embedded in the spinal cord can cause swelling and secondary damage). The vertebral canal remains intact. The main limitation is that the hemisection can only be performed in the intervertebral spaces. 
The results show that this technique can be performed much faster than the traditional surgical approach, using laminectomy (11 min vs. 35 min). This technique can be useful for researchers working with animal models of open spinal cord injury as it is widely adaptable and does not require any additional specialized instrumentation.

Following the hemisection, the rats show paralysis in the ipsilateral hindlimb (in vivo proof of successful hemisection). Thorough specimen evaluation can only be done following the removal of the spinal cord (see Figure 2, where the removed spinal cord can be seen from both the ventral and dorsal sides).
<img alt="Figure 2" class="xfigimg" src="/files/ftp_upload/63534/63534fig02.jpg" /> 
Figure 2...

Watch this Scientific Journal Video about A Minimally Invasive, Fast Spinal Cord Lateral Hemisection Technique for Modeling Open Spinal Cord Injuries in Rats at JoVE.com

A Minimally Invasive, Fast Spinal Cord Lateral Hemisection Technique for Modeling Open Spinal Cord Injuries in Rats

Here, we describe a new, fast technique modeling open spinal cord injury in rats that eliminates laminectomy. Lateral hemisection is performed while viewing through a microscope. The technique is versatile and can also be used in the cervical, thoracic, and lumbar regions of the spinal cord of other animals.

Open spinal cord injury techniques modeling laceration-like injuries are time-consuming and invasive because they involve laminectomy. This new technique ...

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