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DOI :
10.3791/63541-v
June 14th, 2022
Chapters
0:05
Introduction
1:05
Preparation of LB-Agar Plates with Plasmid DNA Transformed Cells and Site-Directed Mutagenesis
1:52
Transformation of Bacterial Cells
3:12
Expression and Protein Purification
6:22
SPR Spectroscopy
7:17
SPR Binding Assay for Cyanovirin-N Binding to Hemagglutinin, S Protein, and RBD
9:56
Results: SPR Binding Assays to Examine Cyanovirin-N Binding to Hemagglutinin and Mannosylated Peptides
11:55
Conclusion
本プロトコルは、HA、S糖タンパク質、関連するハイブリッド型グリカン、および高マンノースオリゴ糖へのCV-N結合を調べるためのSPR結合アッセイのための新しいツールについて説明しています。SPRは、これらのグリカンに二量体または単量体CV-Nのいずれかを結合するためのKD を決定するために使用される。
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