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DOI :
10.3791/63550-v
•
11:30 min
September 16th, 2022
Chapters
0:05
Introduction
2:01
Culturing Cancer Urothelial Cells and Isolation of EVs
6:19
Fracturing of EVs and Making Replicas
8:25
Cleaning Replicas and Replica Analysis
9:47
Results
10:54
Conclusion
我们提出了一种用于分离和冷冻压裂来自癌性尿路上皮细胞的细胞外囊泡(EV)的方案。冷冻断裂技术揭示了EV的直径和形状,并且作为一个独特的特征 ,EV膜的内部组织。这些对于了解电动汽车如何与受体膜相互作用非常重要。
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