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4.7K Views
•
08:22 min
March 23rd, 2022
DOI :
10.3791/63554-v
Chapters
0:05
Introduction
0:52
Maintenance of Murine Small Intestinal Organoids
1:55
Co-Culture of Small Intestinal Organoids with Innate Lymphoid Cells
5:36
Results: Establishing Co-Cultures Between Intestinal Epithelial Cells and ILC1
7:46
Conclusion
Transcript
该协议为研究肠道免疫细胞与上皮之间的相互作用以及剖析这些相互作用如何调节肠道稳态和炎症提供了重要工具。该技术的主要优点是通过体外还原模型促进的精细的实验控制量,可用于解决上皮和免疫生物学中的问题。该系统已被用于确定ILC1在CD44阳性上皮隐窝扩张中的作用,并有可能进一步推进我们对炎症介导的胃肠道疾病的理解。
首先,将热交联基底细胞外基质放在冰上,通过将标准组织培养处理的24孔板置于37摄氏度的培养箱中来解冻和预热。然后,从培养箱中取出含有类器官的板,并从要通过的孔中丢弃培养基
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Summary
该协议为建立小鼠小肠类器官,从小鼠小肠固有层中分离1型先天淋巴细胞,以及在两种细胞类型之间建立3维(3D)共培养物以研究肠上皮细胞和1型先天淋巴细胞之间的双向相互作用提供了详细的说明。
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