Name: rapid genotyping of mouse tissue using sigma's extract-n-amp tissue pcr kit
Uploaded: 2008-01-22T18:40:00
Duration: 12 min 39 s
Description: Watch this Scientific Journal Video about Rapid Genotyping of Mouse Tissue Using Sigma's Extract-N-Amp Tissue PCR Kit at JoVE.com

请<a href="http://www.sigmaaldrich.com/life-science/molecular-biology/dna-and-rna-purification/sybr-green-extractnamp-tissue.html?cm_mmc=JoVE-_-video-_-extract-_-video">点击这里</a>查看协议。

The authors have nothing to disclose.

<table border="1">
	<tbody>
		<tr>
			<td>Material Name</td>
			<td>Type</td>
			<td>Company</td>
			<td>Catalog Number</td>
			<td>Comment</td>
		</tr>
		<tr>
			<td><a href="http://www.sigmaaldrich.com/catalog/ProductDetail.do?lang=en&N4=XNATG|SIGMA&N5=SEARCH_CONCAT_PNO|BRAND_KEY&F=SPEC&cm_sp=affiliate-_-Jove-_-12346">SYBR Green Extract-N-Amp Tissue PCR Kit</a></td>
			<td>Reagent Kit</td>
			<td>Sigma</td>
			<td><a href="http://www.sigmaaldrich.com/catalog/ProductDetail.do?lang=en&N4=XNATG|SIGMA&N5=SEARCH_CONCAT_PNO|BRAND_KEY&F=SPEC&cm_sp=affiliate-_-Jove-_-12346">XNATG</a></td>
			<td>Includes: Extraction Solution Neutralization Solution B Tissue Preparation Solution Extract-N-Amp SYBR Green PCR ReadyMix</td>
		</tr>
	</tbody>
</table>

rapid genotyping of mouse tissue using sigma's extract-n-amp tissue pcr kit

通过PCR扩增和电泳凝胶上检测DNA基因检测是一种标准的方式，确定了组织样本的基因型。常规制备DNA PCR准备组织往往需要几个小时到几天，取决于组织样本。样品的基因型可能因此被推迟了好几天，这是不为许多不同类型的实验的的选项。在这里，我们展示了完整的基因分型鼠尾样品，包括组织消化和PCR读出，在一个和一个半小时，使用Sigma的SYBR GREEN提取物 - N放大器组织PCR试剂盒。首先，我们展示了15分钟的组织样本中的DNA提取。然后，我们展示的样品进行PCR扩增，允许确定一个积极的范例，因为它是被放大的实时读出。一起，快速提取和实时​​读数可以迅速识别的一种可靠的方法通过PCR的各种组织不同类型的基因型。

Watch this Scientific Journal Video about Rapid Genotyping of Mouse Tissue Using Sigma's Extract-N-Amp Tissue PCR Kit at JoVE.com

Rapid Genotyping of Mouse Tissue Using Sigma's Extract-N-Amp Tissue PCR Kit

鼠尾样品的完整的基因分型，包括组织消化和PCR读数，是在一个和一个半小时使用Sigma的SYBR绿色提取N -安培组织PCR试剂盒。

使用Sigma的提取物N -安培组织PCR试剂盒小鼠组织的快速基因分型

Genomic detection of DNA via PCR amplification and detection on an electrophoretic gel is a standard way that the genotype of a tissue sample is ...

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