Name: rapid genotyping of mouse tissue using sigma's extract-n-amp tissue pcr kit
Uploaded: 2008-01-22T18:40:00
Duration: 12 min 39 s
Description: Watch this Scientific Journal Video about Rapid Genotyping of Mouse Tissue Using Sigma's Extract-N-Amp Tissue PCR Kit at JoVE.com

してください<a href="http://www.sigmaaldrich.com/life-science/molecular-biology/dna-and-rna-purification/sybr-green-extractnamp-tissue.html?cm_mmc=JoVE-_-video-_-extract-_-video">ここをクリックして</a>プロトコルを表示します。

The authors have nothing to disclose.

<table border="1">
	<tbody>
		<tr>
			<td>Material Name</td>
			<td>Type</td>
			<td>Company</td>
			<td>Catalog Number</td>
			<td>Comment</td>
		</tr>
		<tr>
			<td><a href="http://www.sigmaaldrich.com/catalog/ProductDetail.do?lang=en&N4=XNATG|SIGMA&N5=SEARCH_CONCAT_PNO|BRAND_KEY&F=SPEC&cm_sp=affiliate-_-Jove-_-12346">SYBR Green Extract-N-Amp Tissue PCR Kit</a></td>
			<td>Reagent Kit</td>
			<td>Sigma</td>
			<td><a href="http://www.sigmaaldrich.com/catalog/ProductDetail.do?lang=en&N4=XNATG|SIGMA&N5=SEARCH_CONCAT_PNO|BRAND_KEY&F=SPEC&cm_sp=affiliate-_-Jove-_-12346">XNATG</a></td>
			<td>Includes: Extraction Solution Neutralization Solution B Tissue Preparation Solution Extract-N-Amp SYBR Green PCR ReadyMix</td>
		</tr>
	</tbody>
</table>

rapid genotyping of mouse tissue using sigma's extract-n-amp tissue pcr kit

電気泳動ゲル上でPCR増幅と検出を介してDNAのゲノム検出は、組織サンプルの遺伝子型が決定される標準的な方法です。 PCR用DNAのための組織の従来の調製は、しばしば組織サンプルに応じて、日に数時間かかる。サンプルの遺伝子型は、このように実験の多くの異なる種類のオプションではない、数日間遅れることがあります。ここでは、組織の消化とPCRの読み出し、いずれかで、シグマのSYBRグリーンエキス- N - Ampの組織PCRキットを使って半時間も含め、マウス尾サンプルの完全な遺伝子型を示す。最初に、我々は、組織サンプルからのDNAの15分の抽出を示す。その後、我々はそれが増幅されていると肯定的なサンプルの識別を可能にするサンプルのPCR増幅のリアルタイム読み出しを示す。一緒に、迅速な抽出とリアルタイムの読み出しは、PCRの確実な方法を通じて、組織の様々な異なる種類の遺伝子型の迅速な同定が可能になります。

Watch this Scientific Journal Video about Rapid Genotyping of Mouse Tissue Using Sigma's Extract-N-Amp Tissue PCR Kit at JoVE.com

Rapid Genotyping of Mouse Tissue Using Sigma's Extract-N-Amp Tissue PCR Kit

組織の消化とPCRの読み出しを含むマウスの尾のサンプルの完全な遺伝子型は、、1とシグマのSYBRグリーンエキス- N - Ampの組織PCRキットを使って半時間で行われます。

シグマのエキス- N - Ampの組織PCRキットを使用してマウス組織の急速なジェノタイピング

Genomic detection of DNA via PCR amplification and detection on an electrophoretic gel is a standard way that the genotype of a tissue sample is ...

Research

JoVE Journal

Biology

Actin Co-Sedimentation Assay; for the Analysis of Protein Binding to F-Actin

アクチン共沈降アッセイ、F -アクチンへの結合タンパク質の解析のための

The actin cytoskeleton within the cell is a network of actin filaments that allows the movement of cells and cellular processes, and that generates ...

Standardized Preparation of Single-Cell Suspensions from Mouse Lung Tissue using the gentleMACS Dissociator

gentleMACS Dissociatorを使用してマウスの肺組織からの単細胞懸濁液の標準化の準備

The preparation of single-cell suspensions from tissues is an important prerequisite for many experiments in cellular research. The process of ...

Rapid PCR Thermocycling using Microscale Thermal Convection

ミクロ熱対流を使用して迅速なPCRの熱サイクル

Many molecular biology assays depend in some way on the polymerase chain reaction (PCR) to amplify an initially dilute target DNA sample to a detectable ...

Chromatin Immunoprecipitation Assay for Tissue-specific Genes using Early-stage Mouse Embryos

初期段階のマウス胚を用いて組織特異的遺伝子のためのクロマチン免疫沈降アッセイ

Chromatin immunoprecipitation (ChIP) is a powerful tool to identify protein:chromatin interactions that occur in the context of living cells 1-3. This ...

A System for Culturing Iris Pigment Epithelial Cells to Study Lens Regeneration in Newt

イモリのレンズ再生を研究するために培養虹彩色素上皮細胞のためのシステム

Salamanders like newt and axolotl possess the ability to regenerate many of its lost body parts such as limbs, the tail with spinal cord, eye, brain, ...

Quantitative Comparison of cis-Regulatory Element (CRE) Activities in Transgenic Drosophila melanogaster

Quantitative Comparison of cis-Regulatory Element CRE Activities in Transgenic Drosophila melanogaster

の定量的比較シス</emトランスジェニックで> -調節エレメント（CRE）の活動キイロショウジョウバエ</em

Gene expression patterns are specified by cis-regulatory element (CRE) sequences, which are also called enhancers or cis-regulatory modules. A typical CRE ...

Mouse Sperm Cryopreservation and Recovery using the I&middot;Cryo Kit

Mouse Sperm Cryopreservation and Recovery using the IÃƒâ€šÃ‚Â·Cryo Kit

I ·クライオキットを使用してマウスの精子の凍結保存と回復

Thousands of new genetically modified (GM) strains of mice have been created since the advent of transgenesis and knockout technologies.  Many of these ...

Rapid and Efficient Zebrafish Genotyping Using PCR with High-resolution Melt Analysis

高解像度メルト解析を用いたPCRを使用して、迅速かつ効率的なゼブラフィッシュ遺伝子型決定

Zebrafish is a powerful vertebrate model system for studying development, modeling disease, and performing drug screening. Recently a variety of genetic ...

qPCRTag Analysis - A High Throughput, Real Time PCR Assay for Sc2.0 Genotyping

qPCRTag分析 - 高スループット、Sc2.0ジェノタイピングのためのリアルタイムPCRアッセイ

The Synthetic Yeast Genome Project (Sc2.0) aims to build 16 designer yeast chromosomes and combine them into a single yeast cell. To date one synthetic ...

Dissection of the Auditory Bulla in Postnatal Mice: Isolation of the Middle Ear Bones and Histological Analysis

生後マウスにおける聴覚ブラの解剖：中耳の骨の単離および組織学的解析

In most mammals, auditory ossicles in the middle ear, including the malleus, incus and stapes, are the smallest bones. In mice, a bony structure called ...