Name: rapid genotyping of mouse tissue using sigma's extract-n-amp tissue pcr kit
Uploaded: 2008-01-22T18:40:00
Duration: 12 min 39 s
Description: Watch this Scientific Journal Video about Rapid Genotyping of Mouse Tissue Using Sigma's Extract-N-Amp Tissue PCR Kit at JoVE.com

Lütfen <a href="http://www.sigmaaldrich.com/life-science/molecular-biology/dna-and-rna-purification/sybr-green-extractnamp-tissue.html?cm_mmc=JoVE-_-video-_-extract-_-video">burada</a> protokol görüntülemek için <a href="http://www.sigmaaldrich.com/life-science/molecular-biology/dna-and-rna-purification/sybr-green-extractnamp-tissue.html?cm_mmc=JoVE-_-video-_-extract-_-video">tıklayın</a> .

The authors have nothing to disclose.

<table border="1">
	<tbody>
		<tr>
			<td>Material Name</td>
			<td>Type</td>
			<td>Company</td>
			<td>Catalog Number</td>
			<td>Comment</td>
		</tr>
		<tr>
			<td><a href="http://www.sigmaaldrich.com/catalog/ProductDetail.do?lang=en&N4=XNATG|SIGMA&N5=SEARCH_CONCAT_PNO|BRAND_KEY&F=SPEC&cm_sp=affiliate-_-Jove-_-12346">SYBR Green Extract-N-Amp Tissue PCR Kit</a></td>
			<td>Reagent Kit</td>
			<td>Sigma</td>
			<td><a href="http://www.sigmaaldrich.com/catalog/ProductDetail.do?lang=en&N4=XNATG|SIGMA&N5=SEARCH_CONCAT_PNO|BRAND_KEY&F=SPEC&cm_sp=affiliate-_-Jove-_-12346">XNATG</a></td>
			<td>Includes: Extraction Solution Neutralization Solution B Tissue Preparation Solution Extract-N-Amp SYBR Green PCR ReadyMix</td>
		</tr>
	</tbody>
</table>

rapid genotyping of mouse tissue using sigma's extract-n-amp tissue pcr kit

Genomik DNA&#39;nın elektroforetik bir jel üzerinde PCR ve algılama yoluyla algılama, bir doku örneği genotip kararlı olduğu bir standart yoludur. Hazır PCR-DNA dokuların Konvansiyonel hazırlık genellikle doku örneği bağlı olarak, gün birkaç saat sürebilir. Böylece örnek genotip deney birçok farklı türleri için bir seçenek değildir, birkaç gün süreyle ertelendi olabilir. Burada doku sindirim ve PCR okuma, bir ve Sigma&#39;nın SYBR Green Özü-N-Amp Doku PCR Kit kullanarak bir buçuk saat dahil olmak üzere, bir fare kuyruğu örnek tam genotipleme göstermektedir. İlk olarak, DNA doku örneği onbeş dakikalık çıkarma göstermektedir. Sonra, biz amplifiye olarak tanımlanması için olumlu bir örnek verir örnek PCR, gerçek zamanlı okuma göstermektedir. Birlikte çıkarma ve gerçek zamanlı okuma, hızlı, güvenilir PCR yöntemi ile dokuların çeşitli farklı türde genotip bir istemi tanımlanması için izin verir.

Watch this Scientific Journal Video about Rapid Genotyping of Mouse Tissue Using Sigma's Extract-N-Amp Tissue PCR Kit at JoVE.com

Rapid Genotyping of Mouse Tissue Using Sigma's Extract-N-Amp Tissue PCR Kit

Doku sindirim ve PCR okuma da dahil olmak üzere bir fare kuyruğu örnek tam genotipleme, bir ve bir buçuk saat Sigma&#39;nın SYBR Green Özü-N-Amp Doku PCR kiti kullanılarak yapılır.

Sigma&#39;nın Özü-N-Amp Doku PCR Kit kullanarak Fare Doku hızlı Genotiplendirme

Genomic detection of DNA via PCR amplification and detection on an electrophoretic gel is a standard way that the genotype of a tissue sample is ...

Research

JoVE Journal

Biology

Actin Co-Sedimentation Assay; for the Analysis of Protein Binding to F-Actin

Aktin Co-Sedimantasyon Testi; F-Aktin Bağlayıcı Protein Analizi

The actin cytoskeleton within the cell is a network of actin filaments that allows the movement of cells and cellular processes, and that generates ...

Standardized Preparation of Single-Cell Suspensions from Mouse Lung Tissue using the gentleMACS Dissociator

Standardize Hazırlık gentleMACS Dissociator kullanarak Fare Akciğer Doku Tek Hücre Cezalar

The preparation of single-cell suspensions from tissues is an important prerequisite for many experiments in cellular research. The process of ...

Rapid PCR Thermocycling using Microscale Thermal Convection

Mikro Termal Isı Yayımı kullanarak Rapid PCR Thermocycling

Many molecular biology assays depend in some way on the polymerase chain reaction (PCR) to amplify an initially dilute target DNA sample to a detectable ...

Chromatin Immunoprecipitation Assay for Tissue-specific Genes using Early-stage Mouse Embryos

Erken evre Fare Embriyolar kullanarak Doku özel Genler Kromatin Immunoprecipitation Testi

Chromatin immunoprecipitation (ChIP) is a powerful tool to identify protein:chromatin interactions that occur in the context of living cells 1-3. This ...

A System for Culturing Iris Pigment Epithelial Cells to Study Lens Regeneration in Newt

Newt Lens Rejenerasyon Eğitim Kültürleme İris Pigment Epitel Hücreler için Sistem

Salamanders like newt and axolotl possess the ability to regenerate many of its lost body parts such as limbs, the tail with spinal cord, eye, brain, ...

Quantitative Comparison of cis-Regulatory Element (CRE) Activities in Transgenic Drosophila melanogaster

Quantitative Comparison of cis-Regulatory Element CRE Activities in Transgenic Drosophila melanogaster

Uygulamalarda Kantitatif Karşılaştırılması Cis</emTransgenik>-Regulatory Element (CRE) Etkinlikler Drosophila melanogaster</em

Gene expression patterns are specified by cis-regulatory element (CRE) sequences, which are also called enhancers or cis-regulatory modules. A typical CRE ...

Mouse Sperm Cryopreservation and Recovery using the I&middot;Cryo Kit

Mouse Sperm Cryopreservation and Recovery using the IÃƒâ€šÃ‚Â·Cryo Kit

I · Cryo Kit kullanarak Fare Sperm Kriyoprezervasyon ve Kurtarma

Thousands of new genetically modified (GM) strains of mice have been created since the advent of transgenesis and knockout technologies.  Many of these ...

Rapid and Efficient Zebrafish Genotyping Using PCR with High-resolution Melt Analysis

Yüksek çözünürlüklü Melt Analizi ile PCR kullanarak hızlı ve verimli Zebra Genotiplemesi

Zebrafish is a powerful vertebrate model system for studying development, modeling disease, and performing drug screening. Recently a variety of genetic ...

qPCRTag Analysis - A High Throughput, Real Time PCR Assay for Sc2.0 Genotyping

qPCRTag Analizi - A Yüksek Verimli, Sc2.0 Genotiplendirme için Real Time PCR Testi

The Synthetic Yeast Genome Project (Sc2.0) aims to build 16 designer yeast chromosomes and combine them into a single yeast cell. To date one synthetic ...

Dissection of the Auditory Bulla in Postnatal Mice: Isolation of the Middle Ear Bones and Histological Analysis

Doğum sonrası Farelerde İşitsel Bulla diseksiyonu: Orta Kulak Kemikler ve histolojik analiz izolasyonu

In most mammals, auditory ossicles in the middle ear, including the malleus, incus and stapes, are the smallest bones. In mice, a bony structure called ...