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DOI :
10.3791/63655-v
April 1st, 2022
Chapters
0:04
Introduction
0:49
Isolation of Nuclei and Primary Antibody Binding
2:40
Binding of pAG-MNase to Antibody
3:36
Library Preparation for Sequencing
5:22
CUT&RUN Sequence Analysis
5:54
Results: Quality Control Steps and Evaluation of Ndt80 and Efg1 Enriched Peaks Using CUT&RUN Protocol
7:22
Conclusion
该协议描述了一种实验方法和数据分析工作流程,用于在靶标下切割并使用核酸酶(CUT&RUN)在人类真菌病原体 白色念珠菌中释放。
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