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<table border="1" fo:keep-together.within-page="1" fo:keep-with-next.within-page="always">
	<tbody>
		<tr>
			<td>488 laser</td>
			<td>Melles-Griot</td>
			<td>-</td>
			<td>CW&#160; laser 50 mW</td>
		</tr>
		<tr>
			<td>60x PLAPON oil immersion objective (NA 1.42)</td>
			<td>Olympus</td>
			<td>1-U2B933</td>
			<td>Lens Confocal microscope</td>
		</tr>
		<tr>
			<td>633 laser</td>
			<td>Melles-Griot</td>
			<td>-</td>
			<td>CW He-Ne laser 12 mW</td>
		</tr>
		<tr>
			<td>63x objective (NA 1.40, HC PL APO CS2)</td>
			<td>Leica</td>
			<td>11506350</td>
			<td>Lens Two-photon microscope</td>
		</tr>
		<tr>
			<td>BA 410-460 nm</td>
			<td>Chroma</td>
			<td>-</td>
			<td>Band-pass filter</td>
		</tr>
		<tr>
			<td>BA 495-540 nm</td>
			<td>Chroma</td>
			<td>-</td>
			<td>Band-pass filter</td>
		</tr>
		<tr>
			<td>BA 505-605 nm</td>
			<td>Chroma</td>
			<td>-</td>
			<td>Band-pass filter</td>
		</tr>
		<tr>
			<td>BA 655-755 nm</td>
			<td>Chroma</td>
			<td>-</td>
			<td>Band-pass filter</td>
		</tr>
		<tr>
			<td>Boron-dipyrromethane (BODIPY) 665/676</td>
			<td>Thermo Fisher Scientific</td>
			<td>B3932</td>
			<td>Lipid peroxiation sensor</td>
		</tr>
		<tr>
			<td>Carbomer-940</td>
			<td>Snowdrift Farm</td>
			<td>739601480651</td>
			<td>Gel</td>
		</tr>
		<tr>
			<td>Catheter</td>
			<td>Terumo</td>
			<td>SV27EL</td>
			<td>Winged infusion sets&#160;</td>
		</tr>
		<tr>
			<td>Cauterizer&#160;</td>
			<td>Braintree Scientific, Inc</td>
			<td>GEM 5917</td>
			<td>Cautery system</td>
		</tr>
		<tr>
			<td>CMV-Cre mouse&#160;</td>
			<td>Jackson lab</td>
			<td>006054</td>
			<td>Mouse line</td>
		</tr>
		<tr>
			<td>Coverslip</td>
			<td>Bioptechs</td>
			<td>-</td>
			<td>30mm diameter coverlip for inverted microscope</td>
		</tr>
		<tr>
			<td>Curity 4x4 inch all purpose sponge gauze</td>
			<td>Covidien</td>
			<td>9024</td>
			<td>Sponge</td>
		</tr>
		<tr>
			<td>EGFPcyto mouse</td>
			<td>Jackson lab</td>
			<td>003291</td>
			<td>Mouse line</td>
		</tr>
		<tr>
			<td>Fiji/ImageJ software</td>
			<td>Open source</td>
			<td>-</td>
			<td>Free software tool</td>
		</tr>
		<tr>
			<td>Fine forceps</td>
			<td>Braintree Scientific, Inc</td>
			<td>FC003 8</td>
			<td>Tissue forceps</td>
		</tr>
		<tr>
			<td>Fluoview 1000 microscope</td>
			<td>Olympus</td>
			<td>FV1000</td>
			<td>Confocal microscope</td>
		</tr>
		<tr>
			<td>FluoView software</td>
			<td>Olympus</td>
			<td>-</td>
			<td>Confocal microscope and Two-photon microscope</td>
		</tr>
		<tr>
			<td>Hand-held electric razor</td>
			<td>Braintree Scientific, Inc</td>
			<td>CLP-8786-451A</td>
			<td>Cordless clipper</td>
		</tr>
		<tr>
			<td>Heat pad</td>
			<td>Braintree Scientific, Inc</td>
			<td>DPIP</td>
			<td>Heat pad for animals</td>
		</tr>
		<tr>
			<td>HyD detectors</td>
			<td>Leica</td>
			<td>-</td>
			<td>Leica 4Tune spectral detector</td>
		</tr>
		<tr>
			<td>Imaris software</td>
			<td>Bitplane / Oxford instruments</td>
			<td>-</td>
			<td>Commercial software tool</td>
		</tr>
		<tr>
			<td>Ingisht X3 tunable laser</td>
			<td>Spectra Physics</td>
			<td>Insight X3</td>
			<td>Tunable Pulse-Laser</td>
		</tr>
		<tr>
			<td>Isoflurane</td>
			<td>VetOne</td>
			<td>13985-046-40</td>
			<td>Anesthetic</td>
		</tr>
		<tr>
			<td>Ketamine&#160;</td>
			<td>VetOne</td>
			<td>13985-702-10</td>
			<td>Anesthetic</td>
		</tr>
		<tr>
			<td>LAS X Software</td>
			<td>Leica</td>
			<td>-</td>
			<td>Two-photon microscope software tool</td>
		</tr>
		<tr>
			<td>Mai-Tai tunable laser</td>
			<td>Spectra Physics</td>
			<td>Mai-Tai</td>
			<td>Laser</td>
		</tr>
		<tr>
			<td>MetaMorph</td>
			<td>Molecular Devices</td>
			<td>-</td>
			<td>Commercial software tool</td>
		</tr>
		<tr>
			<td>Monodansylpentane AUTODOT</td>
			<td>Abcepta</td>
			<td>Sm1000a</td>
			<td>Lipid droplet dye</td>
		</tr>
		<tr>
			<td>MPE-RS microscope</td>
			<td>Olympus</td>
			<td>IX70</td>
			<td>Two-photon microscope</td>
		</tr>
		<tr>
			<td>mT/mG mouse</td>
			<td>Jackson lab</td>
			<td>007676</td>
			<td>Mouse line</td>
		</tr>
		<tr>
			<td>Objective heater</td>
			<td>Bioptechs</td>
			<td>150819</td>
			<td>Objective heater for both confocal and two-photon microscopes</td>
		</tr>
		<tr>
			<td>Oxygen-saturated respiration chamber</td>
			<td>Patterson Scientific</td>
			<td>78933385, SAS3 and EVAC4</td>
			<td>Gas Anesthesia and evacuation system&#160;</td>
		</tr>
		<tr>
			<td>Parafilm</td>
			<td>Heathrow Scientific</td>
			<td>HS234526B</td>
			<td>Semi-transparent, flexible, thermoplastic film</td>
		</tr>
		<tr>
			<td>PMT detector</td>
			<td>Olympus</td>
			<td>-</td>
			<td>Descanned&#160;&#160; detectors</td>
		</tr>
		<tr>
			<td>PMT detector</td>
			<td>LSM-Technology</td>
			<td>Custom built</td>
			<td>Non-Descanned Detectors</td>
		</tr>
		<tr>
			<td>Pump</td>
			<td>Harvard Apparatus</td>
			<td>703602, 704402</td>
			<td>Nanomite injector and controller</td>
		</tr>
		<tr>
			<td>Saline</td>
			<td>Quality Biological</td>
			<td>114-055-721EA</td>
			<td>Normal saline</td>
		</tr>
		<tr>
			<td>Sharp blunt-ended scissors</td>
			<td>Braintree Scientific, Inc</td>
			<td>SCT-S 508</td>
			<td>Surgical scissors</td>
		</tr>
		<tr>
			<td>Syringe</td>
			<td>Covidien</td>
			<td>22-257-150</td>
			<td>1mL tuberculin syringe</td>
		</tr>
		<tr>
			<td>TCS SP8 Dive Spectral microscope</td>
			<td>Leica</td>
			<td>SP8</td>
			<td>Two-photon microscope</td>
		</tr>
		<tr>
			<td>Tweezers&#160;</td>
			<td>Braintree Scientific, Inc</td>
			<td>FC032</td>
			<td>Tissue forceps</td>
		</tr>
		<tr>
			<td>Xylazine&#160;</td>
			<td>VetOne</td>
			<td>13985-704-10</td>
			<td>Anesthetic</td>
		</tr>
	</tbody>
</table>

intravital subcellular microscopy of the mammary gland

Watch this Scientific Journal Video about Intravital Subcellular Microscopy of the Mammary Gland at JoVE.com

<meta charset="utf8"></head><body>乳腺活体亚细胞显微镜检查

乳腺活体亚细胞显微镜检查

The mammary gland constitutes a model par excellence for investigating epithelial functions, including tissue remodeling, cell polarity, and secretory ...

intravital-subcellular-microscopy-of-the-mammary-gland

Research

JoVE Journal

Biology

Intravital Subcellular Microscopy of the Mammary Gland

803 Views.  National Institutes of Health. To begin, place the shaved, anesthetized mouse on a warming pad. Pinch the skin with tweezers near the fourth nipple and cut the raised skin with sharp scissors to make a midline incision of approximately one centimeter. Make circular incisions around the gland in both cranial and caudal directions, and carefully peel the skin away from the abdomen.Remove any exogenous blood promptly with physiological saline to avoid subsequent loss of optical resolution. To minimize blood loss, seal ...