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6.5K Views
•
10:12 min
March 23rd, 2022
DOI :
10.3791/63684-v
Chapters
0:04
Introuduction
1:16
Derivation of Human Lung Organoid
3:25
Expansion of Human Lung Organoids
5:31
Proximal Differentiation to Generate Mature Airway Organoids
7:42
Results: Deriving Human Lung Organoids and Proximal Differentiation to Generate 3D and 2D Airway Organoids
9:38
Conclusion
Transcript
我们正在引入一种在培养板中建立人类气道上皮的类器官培养的方法。我们直接从初级肺组织中高效衍生出人肺类器官。衍生的人肺类器官稳定扩张超过一年,近端分化方案允许产生成熟的气道类器官,可以忠实地模拟人气道上皮到接近生理水平。
因此,培养系统允许科学家重建和扩展培养板中的人类气道上皮。演示该程序的将是我们实验室的Man Chun Chiu和Yifei Yu博士生开始从人体肺组织中分离细胞以进行3D类器官培养,用无菌手术刀将肺组织切成一毫米的小块,并用10毫升冷基底培养基洗涤组织块。在4
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Summary
该协议提出了一种从原代肺组织衍生人肺类器官的方法,扩大肺类器官并诱导近端分化以产生3D和2D气道类器官,从而忠实地对人气道上皮进行表型检查。
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